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在第二种制剂中,2gm的水杨酸USP与维生素E醋酸盐(125U/ml)2ml混合,用基础PCCA化妆用HRT(Base PCCACosmetic HRT)(Professional Compounding Centers of America,Inc.,Houston,TX)补齐到100gm。含有SOI的表达载体用无菌水稀释,随后在基础PCCA化妆用HRT中补齐为10ml,至最终浓度为0.01%活性成分重量比体积,并且用注射管到...
21.将连接产物转入20倍体积的感受态大肠杆菌中,加入100倍连接产物体积的lb培养基,37℃、180r/min摇床培养45min;取适量菌液在涂布在含有氨苄青霉素的平板上,37℃、180r/min恒温箱过夜培养,平板上挑菌接种至含有氨苄青霉素的lb培养基中,37℃、180r/min摇床过夜培养,获得含pet22b-circligase的大肠杆菌e.coil dh5α...
CircLigase ssDNA Ligase产品说明书 Manual CircLigase ssDNA Ligase For Research Use Only. Not for use in diagnostic procedures.CircLigase™ ssDNA Ligase is part of the Epicentre™ product line,known for its unique genomics kits, enzymes, and reagents which offer high quality and reliable performance...
Our results showed that primer ratio (sense primer:antisense primer) of 20:1 and sense primer amountof 10 to 100 pmol, up to 20 PCR cycles using 20 ng of initial template, in combination with polyacrylamide gel electrophoresis,were the optimal conditions for generating good quality and quantity...
The proteins pulled down with biotin-labeled ssDNA donors were subjected to SDS-PAGE. After electrophoresis, the gel was washed with deionized water and covered with fix buffer (50% Methanol, 12% HAC, 0.05% Formaldehyde) for 2 h under slow rotation. Then the gel was washed with wash buff...
Exonuclease I (0.6 U/ µL, New England Biolabs) was mixed with the purified DNA (20 ng/µL) in rCutSmart buffer and incubated at 37 °C for 5 min. The reaction mix was directly used for electrophoresis using 2% agarose gel. Evaluation of the strand selectivity of...
a, Coomassie staining of the purified trimeric human RPA complex separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). b, Spontaneous formation of spherical liquid droplets and surface wetting by the purified RPA complex. Representative stills from time-lapse microscopy are...
(ssDNAs) serve as very efficient donors, both for insertion and for gene replacement. We call this method efficient additions with ssDNA inserts–CRISPR (Easi-CRISPR) because it is a highly efficient technology (efficiency is typically 30–60% and reaches as high as 100% in some cases). ...
Plasmids pCANTAB6_H107Y, pCANTAB6_T108R and pCANTAB6_D116G were isolated, digested with SfiI and NotI and the DNA fragments separated by elec- trophoresis in agarose gel. Each DNA band of 400 bp containing a VHH gene variant was purified and ligated, to the SfiI/NotI-digested pEHLYA5 ...