In co-electroporation experiments we used the same amount of total DNA (i.e., the concentration of the individual plasmids was 0.5 μg/μl). Each embryo was placed between tweezers-type electrodes (CUY650-P5, NEPA Gene). The angle of inclination of the electrode paddles with respect ...
(b) GST-RPEL was used as a bait for pull down of Ddx19 and Ipoβ from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25–10 μM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Full size image...