In co-electroporation experiments we used the same amount of total DNA (i.e., the concentration of the individual plasmids was 0.5 μg/μl). Each embryo was placed between tweezers-type electrodes (CUY650-P5, NEPA Gene). The angle of inclination of the electrode paddles with respect ...
(Polyplus transfection), according to the manufacturer’s instructions. In total, 200 ng of DNA was used for every transfection reaction, with the experimental vector amount varying from 25 to 50 ng depending on the application. The pEF-vector was used to fill up the total amount of DNA...