(nucleophosmin IP and WB) from Abcam; Gsk3β, CST9315 (Gsk3β IP) from Cell Signaling Technology, sc81462 (Gsk3β WB) from Santa Cruz Biotechnology; antibody against C3G (C3G WB), sc-15359 from Santa Cruz Biotechnology; antibody against Tyr(P)-514 of C3G isoforms (Tyr(P)-514-C...
Immunofluorescence, histology, immunohistochemistry and TUNEL assay. Assay was performed as previously described.14 Soft agar assay, Annexin V/7AAD analysis, mitochondrial membrane potential analysis and Src tyrosine kinase activity assay. Anchorage-independent growth was assessed using the colony formation ...
p-JAK2(Y1007/1008) antibody for immunofluorescence analysis was purchased from Abcam. Cell culture The human breast cancer cells MDA-MB 468, BT20, MDA-MB 231, MCF7, T47D, SK-BR-3, HEK-293 and mouse 4T1 breast cancer cells were obtained from the American Type Culture Collection. MDA-...
Approximately 500 μl supernatant in each 1 ml EP tubes was incubated with 1.8 μg of anti-MOR (Abcam), anti-SRC (Cell Signal ing Technology) or anti-p-EGFR (Y854) (Cell Signaling Technology) antibody on rolling stacks at 4 °C for 5 h, meanwhile, 1.8 μg purified rabbit or mouse...
Immunofluorescence staining Fixed cells were permeabilized with 0.1% Triton X-100 for 2 min and incubated in 2% bovine serum albumin (BSA)/PBS at RT to block non-specific interactions. Total RBC were then stained with Alexa555 conjugated α-rabbit antibody (1:500, Invitrogen). Alternatively,...
Slides were incubated overnight at 4 °C with a monoclonal rabbit anti-LPP antibody (EPR6478) (1:750 dilution, Cat. #: ab126608; abcam) or a polyclonal rabbit anti-c-ErbB2 antibody (1:750 dilution, Cat. #: A0485; Dako). Following incubation with the primary antibodies, secondary ...
To determine the localization of hnRNP K in ES(+/+) and ES(-/-) cells, immunofluorescence with an anti-hnRNP K antibody was employed (Fig. 7A). In both, ES(+/+) and ES(-/-) cells, hnRNP K was clearly localized to the nucleus. In HeLa cells cotransfected with plasmids coding ...
Anti-GFP antibody was from Abcam (13970), 1:1000. Anti-RFP antibody was from MBL (PM005), 1:1000. Anti-phospho-CDK1 Tyr15 antibody was from Cell Signaling (4539) used at 1:50. Anti-phospho-MLC2 Thr18/Ser19 antibody was from Cell Signaling (3674), 1:50. Anti-phospho-Histone H3...
Antibodies for western blotting were as follows: anti-SNIP25 interacting protein antibody (Abcam ab111343, USA), anti-phospho-SRC family (Y416) and anti-phospho-SRC (Y527) were purchased from Cell Signaling Technology (#6943 and #2105, respectively; CST,USA), antibodies against SRC, VEGF ...
(e,f) CAL-1 cells were stimulated with R848 for the indicated time periods (minutes) and protein immunoprecipitation was performed using anti-LYN (e) or anti-FYN (f) antibodies or isotype control antibody. Levels of LYN or FYN activating tyrosine phosphorylation and total LYN or FYN were ...