and MTs (time accuracy of 0.2 min) with metabolite standards. For quantification, to correct for the loss of analyte during sample preparation, we used internal standardization technique and then quantification was performed by comparing their peaks against a calibration curves generated using 513 ...
we utilized mutant mice carrying a hepatocyte-specific knockout ofAtg7, an essential gene for autophagy (Atg7f/f;Alb-Cre) and liver-specificAtg7- andNrf2-double-deficient mice (Atg7f/f;Nrf2f/f;Alb-Cre)7,32. Loss of Atg7 caused not only inhibition of conversion of LC3-I to LC3-II...