We determined the concentration of the phage using the double-layer plate method57 and diluted the phage solution to a concentration of 108 in PBS. For the surface-associated growth experiments, we mixed the R388 donor and potential recipient at a ratio of one (cell number:cell number) and ...
The method can be applied to the Huygens-Fresnel principle and can be extended to Rayleigh-Sommerfeld or Helmholtz diffraction and to electromagnetic fields. A complete focused spherical wave converges onto the focal point and then expands outward, giving a focal field of U=Asink0r/r, where A...
After the treatment, the cells were washed twice with phosphate-buffered saline (PBS), trypsinized for 5 min at 37 °C, and lifted from the plate. The cells were collected and analyzed using an Attune™ NxT Flow Cytometer (Thermo Fisher). Total of 10,000 events were recorded per sample...
(perhaps call them methods of science rather than "The Scientific Method.") Hypothesis / experiment / conclusion is one of these, and it's very important in experimental science such as physics and chemistry, but it's certainly not the only method. It would be a mistake to elevate it ...
1. Place the V bracket around the pole, sliding its two grooves up into the aluminum plate on the back of the radio. 2. Once the grooves reach the stops, manually tighten the L shaped bolt so that it "bites" into the pole. Pole Mounting Bracket SI...
ScenariosHRR (MW)Construction shaft length (m)Fire locationDrainage measureSchematic diagram 1 10 6 P1 no 2 10 12 P1 no 3 10 12 P2 no 4 10 18 P2 no 5 10 6 P1 Normal vertical plate S1 6 10 6 P1 Higher vertical plate S2 7 10 12 P1 Normal vertical plate S1 8 10 12 ...
Multi-step growth curves.DEF cells in a 24-well plate were infected with BAC-CHv, BAC-CHv-UL49R, and BAC-CHv-ΔUL49 at an MOI of 0.01. The supernatant and cell samples were collected at the specified time points, respectively, and viral titers were measured. Data were calculated as ...
TP distribution (the Wadati diagram, see Supplementary Fig. 1). This starting pseudo-1D model (Fig. 3a) was preferred to a true minimum 1D model as it represents already a 50% data variance reduction, improving stability and convergence. Inversion method. We used the code Simulps28, which ...
1. A method of tracking a peak of a correlation function in a positioning system receiver, comprising the steps of: sampling a correlation function and obtaining at least four correlation points along the correlation function; generating an error signal based upon a computation of a local maximum...
17.A method of downconverting a first periodic voltage waveform into a second periodic voltage waveform, comprising:providing a control signal indicative of a code used to produce the first voltage waveform;in response to said control signal, obtaining from the first voltage waveform a plurality of...