protein–protein interactionkineticsaffinitysteroidsProtein–protein interactions (PPIs) are crucial for the successful realization of many metabolic and signaling pathways. Of particular interest are PPIs of membrane proteins, which form stable and/or transient complexes for the signal transduction, ion ...
Surface plasmon resonance (SPR) is a widely employed technique for studying protein-protein interactions. Here, we describe a method for the analysis of single-stranded DNA binding protein (SSB)-heterologous protein interactions by SPR. This method avoids several pitfalls often associated with SPR, ...
protein–protein interactionkineticsaffinitysteroidsProtein–protein interactions (PPIs) are crucial for the successful realization of many metabolic and signaling pathways. Of particular interest are PPIs of membrane proteins, which form stable and/or transient complexes for the signal transduction, ion ...
蛋白与脂的相互作用研究也可以使用SPR成像技术。大多数哺乳动物体内均含有CRP蛋白(Creactive protein),CRP蛋白在Ca离子存在下能够与PC(Phosphorylcholine)相互作用。从兔子中提取的CRP蛋白、rCRP是由五个相同的亚单元组成的五元 环,环的一边具有键合PC的位点。虽然大多数细胞膜的脂层中均含有极性的PC基团,但是CRP分子只...
The interaction between 2 and DCTPP1 was detected by SPR using the Biacore S200 system at 25°C (Cytiva). Briefly, recombinant human DCTPP1 protein (MCE, HY-P77508) was immobilized on an activated carboxymethylated 5 (CM5) sensor chip using amine coupling method. Gradient concentrations of ...
Profacgenprovides a comprehensive panel of services for the study of protein-protein interactions, of which theSurface Plasmon Resonance (SPR)analysis is commonly used by our customers for thehighly accurate and sensitive kinetics and affinity measurement. For SPR analysis, the ligand-of-interest is...
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Protein solution of 100 µg ml−1 were added to react with the activated surface. The chip was equilibrated with 1.05x PBS buffer at room temperature for 4 h. Compounds were serially diluted and injected for 60 s (contact phase) at a flow rate of 30 µL min−1, followed by ...
Surface plasmon resonance (SPR) biosensor technology has become an important tool for drug discovery and basic research. SPR instruments are used for a wide variety of applications including determining the binding kinetics and affinity of an interaction
reproducible data. The software supports direct binding assays, which lets you measure active concentration. Calibration-free concentration analysis (CFCA), part of Biacore X100 Plus Package, measures protein concentrations related to specific binding activity, but without having to use a protein standard...