The presence of alloreactive T cells in both the spleen and distal lymph nodes supports their systemic recirculation from the sites of T-cell priming during the process of Lm-mediated loss of tolerance. To assess whether this infection-triggered allograft rejection and increase in alloreactivity in...
To monitor genetically-controlled cytokine-producing ability in mice in vitro, we developed a high-density cell culture system, which is preferable for inducing CD4 + T cell-dependent self-priming responses without any antigenic stimulation. When BALB/c spleen cells were cultured at high density (...
Still, the existence of cell-to-cell heterogeneity in NANOG levels in 2i growth conditions leaves open the question of whether low-NANOG ESCs are undergoing lineage priming or escaping inhibition to enter spontaneous differentiation. In this paper, we used RNA-sequencing and single cell transcript ...
The autorosette forming cells in spleen comprise approximately equal numbers of B and T cells. Whilst autorosette-forming cells in thymus are not affected by anti-Ig treatment those in spleen can be inhibited by capping their Ig. A possible role for self antigens in actively priming immuno...
ICAM-1 contributes to but is not essential for tumor antigen cross-priming and CD8+ T cell-mediated tumor rejection in vivo. ICAM-1-deficient mice successfully rejected HTR.C tumors expressing Ld recognized by the 2C TCR, albeit with a kinetic delay. Inasmuch as HTR.C tumor ... C Blank...
Microglial priming and enhanced reactivity to secondary insult in aging, and traumatic CNS injury, and neurodegenerative disease. Neuropharmacology. 2015;96:29–41. Article PubMed CAS Google Scholar Manich G, Recasens M, Valente T, Almolda B, Gonzalez B, Castellano B. Role of the CD200-CD...
We characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of Tunisia by 16S rDNA polymerase chain reaction (PCR)–restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS...
Systemically, CD200R1-KO mice exhibited signs of persistent infection including lymphopenia, T cell activation and memory conversion, and narrowing of the TCR repertoire. These findings were confirmed in a second model of acute neuroinflammation induced by systemic endotoxin challenge. Conclusion This ...
For experiments involving manipulations of extracellular potassium concentrations, priming was performed in OptiMEM medium and K þ concentrations were increased 30 min before IpaB treatment or S. flexneri infection. Cell death assays. LDH release assay was performed as previously described39 using the...
In particular, the transplantation of tumor cells induces an acute inflammation locally associated with cell death and priming of the immune cell infiltrate17. In contrast, spontaneous tumors emerge from a sterile microenvironment progressively exposed to a chronic inflammation likely to condition ...