Three baths in MiliQ water 5 min each, air dry 1. Incubate sections in 10 mg/ml Lysozime solution (Sigma USA), 0.05 M EDTA, 0.1 M Tris-HCl for 1 h at 37 °C 2. Wash with MiliQ water for 2 min, air dry 1. Incubate sections in 0.1 M HCl solution for ...
Sponges were incubated for a period of 30 min at 37 °C, and then they were rapidly embedded (3 baths by immersion) in a cold interstitial type I collagen solution [7.5 volumes of Collagen R at 2 mg/ml (SERVA Electrophoresis, Heidelberg, Germany), 1 volume of 10x Hanks´ ...