our lab developed the split-ubiquitin based Membrane Yeast Two-Hybrid (MYTH) system. This technology allows for the sensitive detection of transient and stable protein interactions usingSaccharomyces cerevisiaeas a host organism. MYTH takes advantage of the observation that ubiquitin can be sepa...
The split-ubiquitin membrane-based yeast two-hybrid system. Thaminy S,Miller J,Stagljar I. Methods in Molecular Biology . 2004Thaminy S, Miller J, Stagljar I (2004) The split-ubiquitin membrane-based yeast two-hybrid system. Methods Mol Biol 261: 297-312....
It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner. Tags Split-ubiquitin Membrane Yeast Two-hybrid Protein-protein Interactions MYTH System Full-length Transmembrane Proteins Saccharomyces ...
Application of yeast two-hybrid system to plant virology; 酵母双杂交系统在植物病毒学上的应用 3. To screen for proteins interacting with collectrin, the yeast two-hybrid system was performed. 通过酵母双杂交系统从人肾脏cDNA文库中筛选与collectrin相互作用的蛋白 ,可以为该基因的功能研究提供线索 。 更...
two-hybrid system. ODR-10 hetero-oligomerises with STR-112 in the split-ubiquitin yeast two-hybrid system.ODR-10 hetero-oligomerises with STR-112 in the split-ubiquitin yeast two-hybrid system.Muhammad TehseenChunyan LiaoHelen DacresMira Dumancic...
Their split-ubiquitin system (Lehming, 2002) was shown to have advantages beyond previously described technologies such as Y2H and TAP tagging, including the successful identification of membrane and membrane-associated protein–protein interactions (Stagljar et al., 1998). Since the introduction of ...
Functional complementation and transport assays confirmed that in yeast, the two halves of the sucrose transporters form functional carriers at the plasma membrane. Functional reconstitution is not due to stabilization of the complex by the ubiquitin halves, since deletion of both halves of ubiquitin ...
An N-terminal subdomain and a C-terminal subdomain of ubiquitin are linked to a pair of proteins or peptides to be examined for their ability to interact. When contacted with one another, a quasi-native ubiquitin moiety is reconstituted provided that the protein or peptide pair do, in fact...
病害特异蛋白互作的蛋白质 screening of putative proteins in vectorlaodelphax striatellus which are interacted with disease-specific protein ofrice stripe virus by yeast two-hybrid based on the split-ubiquitin.pdf 2015-03-05上传 病害特异蛋白互作的蛋白质 screening of putative proteins in vectorlaodelphax...
Thaminy S, Miller J, Stagljar I (2004) The split-ubiquitin membrane-based yeast two-hybrid system. Methods Mol Biol 261: 297-312.Thaminy S, Miller J, Stagljar I. The split-ubiquitin membrane-based yeast two-hybrid system[J]. Methods Mol Biol, 2004, 261:297-312....