SplintR Ligase Reaction Buffer B0375SVIAL -20 1 x 1 ml 10 X 特性和用法 单位定义 1 单位是指在 20 μl 反应体系中,使用 1X SplintR 连接酶反应缓冲液,在 25℃ 条件下,15 分钟连接(完成 50%)2 pmol 三重 FAM 标记的 DNA:RNA 杂交底物所需的酶量。 1X SplintR 连接酶反应缓冲液 50 mM Tri...
(B) Ligation of the RNA-splinted substrate in SplintR Ligase Reaction Buffer for 15 minutes at25°C with (a) no enzyme, (b) 1 μM T4 DNA Ligase and (c) 100 nM SplintR Ligase. Indicated peaks correspond to starting pDNA (I), AppDNA (II) and ligated product (III) as determined ...
I would like to use SplintR Ligase in a workflow, but not use the provided reaction buffer. Can you tell me what reaction buffer components are needed? Can you tell me what inhibits SplintR Ligase? SplintR Ligase seems very concentrated at 25 units per microliter. How do I know how much ...