Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing. Gene Ther 2000;7:1885-95. [PubMed: 11127576]Mansfield, S. G., et al. (2000). Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing. Gene
RNA repair using spliceosome-mediated RNA trans-splicing. Trends Mol Med. 2004;10:263-268.Mansfield, S.G., Chao, H. and Walsh, C.E. (2004) RNA repair using spli- ceosome-mediated RNA trans-splicing. Trends Mol. Med., 10: 263-268....
The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosomal mediated trans-splicing. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target...
9,10 Splicing-mediated RNA trans-splicing creates a hybrid mRNA from two different molecules through a trans-splicing reaction mediated by the spliceosome that makes it possible to exchange mutated sequences for normal ones. Most of the trans-splicing strategies applied so far have utilized ...
Precursor messenger RNA (pre-mRNA) splicing is an essential step in the flow of information from DNA to protein in all eukaryotes. Research over the past four decades has molecularly delineated the splicing pathway, including characterization of the detailed splicing reaction, definition of the splic...
Precursor messenger RNA (pre-mRNA) splicing is an essential step in the flow of information from DNA to protein in all eukaryotes. Research over the past four decades has molecularly delineated the splicing pathway, including characterization of the detailed splicing reaction, definition of the splic...
近日,上海科技大学刘如娟、西湖大学施一公及圣裘德儿童医院Xu Beisi等团队合作在Nucleic Acids Research 在线发表题为“THUMPD2 catalyzes the N2-methylation of U6 snRNA of the spliceosome catalytic center and regulates pre-mRNA splicing and retinal degeneration ”的研究论文,该研究发现一种RNA修饰-N2 -甲基鸟...
A View to a Kill: Structure of the RNA Exosome Trans-Splicing to Spliceosomal U2 snRNA Suggests Disruption of Branch Site-U2 Pairing during Pre-mRNA Splicing Duncan J. Smith, Charles C. Query, Maria. The Long Unwinding Road of RNA Helicases ...
The studies on the role of the spliceosomal helicases/ATPases in assembly suggest that each ATP-dependent step most likely is mediated by a single family member and these RNA-dependent ATPases contain weak in vitro RNA unwinding activity [3]. The mechanism of nuclear splicing has been compreh...
(g) Spliced leader trans-splicing (SLTS). A short RNA ‘spliced leader’ (SL) is joined to the 5′ end of a protein-coding transcript by a two-step mechanism closely resembling intron splicing. The first step is a nucleophilic attack of the branch point on the mRNA ‘outron’ (the ...