The SPL enzyme reaction mixture contained 50 µL lysate of desired protein amount, 10 µL S1P (13.18 nmol), and 190 µL of reaction assay buffer (50 mM potas- sium phosphate buffer, pH 7.4, 1 mM EDTA, 5 mM DTT, 0.4 mM pyridoxal 5 -phosphate, 100 mM sucrose, 0.1% TritonX-...
aTnhaeloggoyv.erning equation (Equation (6)) can be described as a circuit in theTmheeclhuamnipcaelddpoamraaimnestheorws onfinthFeigmuerech3aunsiicnagl tshyestiemmpe(dMa,nCce, aannadloKg)yw. ere measured using Klippel measuTrehme elunmt epqeduippamraemnte.teKrsliopfptehlemmeeacshuarn...
Sci. 2021, 22, x FOR PEER REVIEW 4 of 19 Int. J. Mol. Sci. 2021, 22, 249 intermediate vector through the Bsa I site were consistent with the designed sgRNA se- quences, so the constructed vector is suitable for the next step of Agrobacterium Mediated genetic transformation of rice (...