To detect protein expression in total cell lysates, cells were lysed in Pierce IP lysis buffer with 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 1 Â proteinase inhibitor cocktail (PIC) (Roche), followed by sodium dodecyl sulfate (SDS)–...
performed at 50 1C for 30 min with 2 mg RNA in a 20-ml reaction volume containing 200 U Superscript III reverse transcriptase (Invitrogen), 500 ng oligo(dT)15 primer (Roche), 0.5 mM dNTP mix (Promega), 5 mM DTT, 40 U RNase inhibitor (RNaseOUT; Life Technologies) in First- Strand...
Centre hospitalier départemental, service de gynécologie obstétrique, 85000 La Roche-sur-Yon, France;Centre hospitalier départemental, service de chirurgie digestive, 85000 La Roche-sur-Yon, France;Perreve, SophieCentre hospitalier départemental, service de gynécologie obstétrique, 85000 La Roche-...
Sojo, Kroonerz, Ron Ractive, Marcel Niveau, Massimiliano Gentile, Andrea Maffei, DJ Thedo, The Dutch Deepartment, Dani Duran & Eleclove, Pbw, Psychokinetic, Emiel Roche, Kephee, Disco Channel, Massimo Gentile, Giuseppe Azul, Slavaki, Fingerspitzen, Showroom Berlin, Benny White, MTM, F. Bot...
Skoko, P. Agostinis, de, Belleroche J., Eriksson, L.A., Fulda, S., Gorman, A.M., Healy, S., Kozlov, A., Muñoz-Pinedo, C., Rehm, M., Chevet, E., Samali, A Endoplasmic reticulum stress signalling - from basic mechanisms to clinical applications FEBS J., 286 (2019), pp...
After 15 h, the protoplasts were lysed in extraction buffer (50 mM Tris-HCl (pH 7.4), 150 mM KCl, 1 mM EDTA, 0.5% Triton-X 100, 1 mM DTT) containing a proteinase inhibitor cocktail (Roche LifeScience). The resulting lysates were incubated with agarose-conjugated anti-Flag antibodies (...
(Roche), 0.5 mM dNTP mix (Promega), 5 mM DTT, 40 U RNase inhibitor (RNaseOUT; Life Technologies) in First-Strand buffer (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2; Life Technologies). Afterwards, 2 μl was used as template for RT-PCR in 20 μl ...
4.8. Urine ACR Analyses Serum and urine albumin and creatinine were measured using the COBAS INTEGRA 400 plus instrument (Roche Diagnostics, Indianapolis, IN, USA) by the University of California Davis Comparative Pathology Laboratory (Davis, CA, USA). 4.9. Quantitative RT-PCR Total RNA was ...