近日,香港科技大学(广州)唐少军团队和清华大学分析中心朱奕颖合作在Research上发表了题为“SARS-CoV-2 Spike Protein Post Translational Modification Landscape and Its Impact on Protein Structure and Function via Computational Prediction”的研究。该研究基于蛋白质组学技术,绘制了全面的SARS-CoV-2spike蛋白的高分辨...
The protein has a calculated MW of 138.0 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. 内毒素(Endotoxin) Less than 1.0 EU perμg by the LAL method. 纯度(Purity) >95% as determined by SDS-PAGE. ...
Among different forms of COVID-19 vaccine design, the spike protein of SARS-CoV-2 virus is widely used as a candidate vaccine antigen. As a surface protein on the virus envelop, the spike was reported to be heavily N-glycosylated and glycosylation had a great impact on its immunogenicity ...
Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein ofsarsCoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein ofsars-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. ...
There is still little information on the molecular processes involved in SARS-CoV-2 spike protein glycosylation and maturation; however, due to the similarity with other CoV spike proteins, such as the SARS-CoV S, the knowledge obtained on these viruses can be used to identify elements of the...
Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. ...
The glycosylation of viral envelope proteins has a wide range of functions, including regulating viral tropism, protein stability and shielding the underlying epitopes from immune surveillance. Thus, a full understanding of the glycosylation of SARS-CoV-2 S protein is critical to reveal the pathogenesi...
分子量: The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to130-140KDa based on Bis-Tris PAGE result. 纯度: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC 内毒素水平: Less than 1 EU per μg by the LAL method. 形态: Lyoph...
Molecular Mass:The recombinant SARS-CoV-2 Spike S1 consists of 678 amino acids and predicts a molecular mass of 76.48 kDa. As a result of glycosylation, it migrates as an approximately 99.56 kDa band in SDS-PAGE under reducing conditions. ...
Mapping glycosylation on the spike protein Like Woods, Parastoo Azadi's previous research—characterizing glycosylation of vaccine candidates for viruses including influenza and HIV—made for a straightforward transition to working on the SARS-CoV-2 spike protein. But unlike Woods, she takes an experime...