TheLEGO EducationSPIKE Prime kit is like having a robot-making course in a box. Very cool! This kit is super helpful for teaching kids in middle school the essentials of coding and building with technology, science, and math, and the unique packaging makes it perfect for homeschoolers. Paren...
Cohorts are color-coded in Figure 1C, and the same color coding is used throughout all subsequent figures. All cohorts were primed with SARS-2 spike to reflect pre-existing SARS-2 immunity. All protein immunizations were adjuvanted with Sigma adjuvant. The control cohort was boosted with wild...
The beta strands in the core-center, the elements in the core-peripheral, and the structures in the external domains are marked with c, p, and the character with prime, respectively. The blue triangle and green star represent the key amino acids for binding hCD26 in MERS-RBD/CTD and HKU...
The SARS-CoV-2 S protein structure (PDB ID: 6VXX) was manually processed via the Schrodinger Maestro Suite with the Protein Preparation Wizard89removing ligands and water molecules, filling missing residues with Prime90, determining protonation states in physiological pH (7.4) with PropKa91and mini...
The S2Pprotein was purified as previously reported24. Briefly, the prefusion S ectodomain, the mammalian codon-optimized gene coding SARS-CoV-2 (Wuhan-Hu-1 strain, GenBank ID: MN908947.3) S glycoprotein ectodomain (residues M1–Q1208) with proline substitutions at K986 and V987, a “GSAS”...
For each sequence, we counted the number of undetermined nucleotides (such as N, Y, W) for whole genomes as well as S genes and obtained 40,739 sequences with fewer than 1,000 undetermined nucleotides in the genome and fewer than 10 undetermined nucleotides in the S-coding region. We ...
110 In brief, HEK293T/17 cells cultured in DMEM supplemented with 10% FBS were transfected with a pcDNA3.1-derived expression plasmid (Invitrogen) coding for the SARS-CoV-2 S protein (GenBank ID: QHD43416.1) with shortened cytoplasmic tail, i.e., pSARS-CoV-2-S-CΔ19, using ...