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To discover peptide activators that can induce pain and recover pain responses in Nav1.7-related CIP, we first fractionated 15 crude venoms (from ten spiders and five snakes) using semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC) and collected a total of 110 fractions...
CryoEM structure determination of α-LCT We recombinantly expressed the mature α-LCT (amino acids 16–1240) fromLatrodectus tredecimguttatus(Mediterranean black widow) (UniprotKB ID: Q9XZC0 (LCTA_LATTR)) (Fig.1aand Supplementary Fig.1) in insect cells using the MultiBac system and purified it...
CryoEM structure determination of α-LCT We recombinantly expressed the mature α-LCT (amino acids 16–1240) fromLatrodectus tredecimguttatus(Mediterranean black widow) (UniprotKB ID: Q9XZC0 (LCTA_LATTR)) (Fig.1aand Supplementary Fig.1) in insect cells using the MultiBac system and purified it...
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Friedel, et al., "Immobilizing and Lethal Effects of Spider Venoms on the Cockroach and the Common Mealbeetle", Toxicon, 27:305-316, 1989. Roth, "The Spider Genus Tegenaria in the Western Hemisphere (Agelenidae)", Amer. Museum Novitates, 2323:1-33, 1968. Vest, "Necrotic Arachnidism ...
In general, the main toxin components of most spider venoms have been well identified as a complex mixture of proteins, polypep- tides, polyamine neurotoxins, nucleic acids, free amino acids, monoamines, and inorganic salts that cause a wide range of effects in both vertebrates and invertebrates...
Spider venoms are complex mixtures of proteins, peptides and non-peptides low molecular mass organic (NPLMM) molecules (Mr < 1 kDa), which act mainly on the nervous system and present a wide range of pharmacological effects on synaptic transmission. The purpose of the present manuscript is no...
Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas. Toxicon 2005, 45, 489–499. 14. da Silveira, R.B.; Chaim, O.M.; Mangili, O.C.; Gremski, W.; Dietrich, C.P.; Nader...