Conclusions: Comparing the results, we concluded that the forced floating method using ULA plates was considered more suitable and straightforward to generate RT4 spheroids for drug screening/cytotoxicity assays. The results presented here also contribute to the improvement in the standardization of the ...
Spheroids were created using different approaches: the hanging drop method, the round bottom wells and the cell-embedded Matrigel. The hanging drop and the round bottom well methods were tested with and without the use of MC in the medium. To prepare MC-enriched medium, complete medium was sup...
Spheroids were made by the hanging-drop method using the MCF-7 cancer cell line. To generate photoacoustic contrast, MCF-7 cells were incubated with optical absorbing nanoparticles (e.g., gold nanorods, 780 nm absorption) for 24 h and mixed with native MCF-7 cells prior to spheroid ...
(1) the gene expression of ECM proteins, their regulators, and ER-stress related factors of human corneal stromal (HCSFs) cells were examined using our recently developed three-dimension (3D) drop culture27,28,29,30,31 in addition to conventional two-dimension (2D) cultures, and (2) on ...
The method has been successfully applied to a broad range of cell lines and shows nearly 100% efficiency (i.e., one spheroid per drop). Using the hepatoma cell line, HepG2, the hanging drop method generated well-rounded MCTS with a narrow size distribution (coefficient of variation [CV] ...
2.4 Comparison of cardiac spheroids formed using the HH-chip, the hanging-drop method, and low-attachment plates We compared three different cardiac spheroid culture methods: our developed method, the conventional hanging drop method, and the low-attachment plate method. After 24 h of culturing,...
采用液滴重叠法(1iquidoverlaymethod)来构建HeLa肿瘤多细胞球模型,并 在光镜下对肿瘤多细胞球的生长状况进行观察描述,再应用场发射扫描电镜和透射电镜观察MCTSs对肿瘤多细胞 球的外部和内部超微结构及双光子激光共聚焦荧光显微镜对活细胞球进行激光层切,建MCTSs的三维立体形貌。 还利用该模型对于表面不同电荷的量子点...
Cell aggregates, including sheets and spheroids, represent a simple yet powerful model system to study both biochemical and biophysical intercellular interactions. However, it is becoming evident that, although the mechanical properties and behavior of multicellular structures share some similarities with in...
Using the microfluidic system, the size of the spheroids can be tuned to between 100 and 130 μm with generation frequencies of 70 Hz. We demonstrated the photothermal therapy (PTT) application of brain tumor spheroids generated by the microfluidic device using a reduced graphene oxide-branched...
Therefore, a design and manufacturing method, which has relatively low cost, high throughput, and high size flexibility, is proposed. Cell culture plate was fabricated by computer aided design and manufacturing software using polydimethylsiloxane as a plate constituent. With the successfully-developed ...