This microscope image provided by Osaka University and the Research Institute of Molecular Pathology in October 2024, shows the fertilization of a mouse egg marked in red and green. DNA is marked in blue, showing in the egg at the top and in a sperm cell at the bottom left. (Yonggang ...
Deep within the darkened CAVE, the giant sperm looms. Its monstrous tail curls around and around, snarled in a series of ferocious knots. Under an ordinary microscope, the tangled tail would be an impenetrable blob. But here in the Cave Automatic Virtual Environment at the National Center ...
Cells growing in culture LIVE/DEAD Sperm Viability Kit (Cat. No. L7011) Fluorescence microscope with FITC and TRITC filters pH 7.4 HEPES buffer, such as Live Cell Imaging Solution (Cat. No. A14291DJ) Bovine serum albumin (BSA) (Cat. No. 15561020) ...
Computer-assisted sperm analysis (CASA) is based on the sperm image, through the image-processing algorithm to detect the position of the sperm target and track tracking, so as to judge the sperm activity. Because of the small and dense sperm targets in sperm images, traditional image-...
Image Type Negative phase contrast for motility, brightfield for DNA fragmentation and morphology Temperature Control Heat from 20°C (Room Temperature + ~3°C) to 45°C Compatible Specimen Chambers Type: 20 micron depth Slide and Makler for motility analysis Objectives Image Type: Dark field, ...
Video image processing greatly enhances contrast, quality, and speed in polarization-based microscopy. Video cameras with contrast and black level controls can yield polarized light and differential interference contrast microscope images with unprecedented ... Inoue,S. - 《Journal of Cell Biology》 被...
Image analysis was applied to photographs of sperm samples obtained using a scanning electron microscope. This technique is too time-consuming for routine use but could, if automated, provide an objective assessment of sperm morphology. In order to test the technique, sperm samples were evaluated fr...
UV light reached the sample through the backport of the microscope and a 380 nm long-pass dichroic filter (380 DCLP; Chroma). Tracking of the flagellum was achieved with custom-made programs written in MATLAB (Mathworks). The program identified the best threshold for binarization of the ...
Full size image Using a transmission electron microscope (Fig. 7A), it was also noted that the sperm head was twisted and that the nucleus resembled a helical shape, which was supported by semen smears stained with acridine orange and studied with a fluorescence microscope (Fig. 7B). Figure ...
Full size image We then tested whether CENTLEIN could interact with PMFBP1 and/or SPATA6. Although SPATA6 could not bind to CENTLEIN (see below), epitope-taggedCentleinandPmfbp1expressed in HEK293T cells were able to interact with each other in reciprocal immunoprecipitation experiments (Fig....