While some proteases likely function to activate sperm motility37, it is possible that the catabolic cocktail in seminal fluid would be detrimental to an unprotected cell. In Drosophila pseudoobscura, a proportion of sperm are killed within 30 min of insemination, likely due to female reproductive...
We assume a dynamic regulation of the shape of the flagellar beat in response to the concentration stimulus s(t) sampled by the cell along its path: sðtÞ ¼ cðrðtÞ; tÞ: ð4Þ The time-dependent stimulus s(t) is transduced by a simple adaptation mod- ule19,65,...
Empty Cell Captive breeding with ART back-cross (every 4 years) 17 0.068 20.1 0.95 21.1 White-bellied frog (Geocrinia alba) Closed captive breeding 400 0.72 284.5 - 284.5 Empty Cell Captive breeding with ART back-cross (every 4 years) 17 0.049 12.4 0.95 13.4 Dibbler (Parantechinus api...
Each group of blastocyst pool was separately collected and performed according to the Cell Amp Whole Transcriptome Amplification Kit (TaKaRa, Japan). cDNA was amplified through 20 cycles of PCR. Subsequently, 10-fold diluted cDNA was used as the template for Q-PCR, which was performed based on...
(Foster and Pizzari2010; Moore et al.2002). In order to reach the egg first, sperm from rodents display a distinct behaviour by which hundreds or thousands of cells aggregate to form a train. These sperm trains swim at a speed that is about 50% higher than that of the individual cell...
transport capacity should be able to significantly change substrate concentrations within a few seconds. SLC transporters feature transport rates of a few hundred substrate molecules/s; thus, even if present, copy numbers in the range of <10-20 molecules/cell could not substantially change concentrati...
ChIP. ChIP was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) with the indicated antibodies. Primers for ChIP-qPCR are listed in Supplementary Table 5. Centrifugal elutriation. Fractionation of spermatogenic cells through centrifugal elutriation was performed as previously described...
transport capacity should be able to significantly change substrate concentrations within a few seconds. SLC transporters feature transport rates of a few hundred substrate molecules/s; thus, even if present, copy numbers in the range of <10-20 molecules/cell could not substantially change concentrati...
(Cell Signaling Technology, Beverly, USA, #9624), anti-phosphotyrosine (Tyr-P) (Abcam, Cambridge, UK, #ab10321) and anti-β-tubulin (β-TUB) (Sigma-Aldrich Quimica S.A., Madrid, Spain, #T0198)78,79. Horseradish peroxidase-conjugated anti-rabbit and anti-mouse IgGs were obtained from ...
A related aspect of the invention provides a method of decreasing Ca+2 influx into a cell, such as in a spermatozoon, the method comprising contacting the cell with a compound represented by formula (I), or any one of formulas (II)-(VI). Another aspect of the invention provides a method...