In fact, SPY-like proteins extensively exist in diverse branches of life, including prokaryotes, protists, algae and all plants, indicating that O-fucosylation by SPY is a common way to regulate intracellular protein functions1. Lacking a structure of full-length SPY and information of how it i...
This finding challenges the view based on posterior Hox protein studies that low-affinity Hox–Exd sites are the rule to ensure Hox protein specificity. Results AP-2 is required for the development of maxillary structures During embryogenesis, AP-2 is expressed in the brain and in the maxillary...
Much has been made of knock-out (KO) validation. It can be a powerful tool to demonstrate protein expression as well as antibody sensitivity. When one knows for certain which cell lines express our target, then a knockout can be used. Unfortunately, it’s not always possible to use a kn...
The Fur protein, which regulates iron uptake in Escherichia coli, also represses the biosynthesis of the manganese-containing superoxide dismutase (MnSOD). A strain of E. coli bearing a lacZ fusion to the aerobactin operon was used to compare the metal specificities of the regulation of MnSOD ...
Bevacizumab, erlotinib, and imatinib in the treatment of patients (pts) with advanced renal cell carcinoma (RCC): A Minnie Pearl Cancer Research Network phase I/II trial Most pts with clear cell RCC have loss of Von Hippel Lindau (VHL) protein primarily due to gene mutation, ultimately result...
Diagnosis of Alzheimer's disease (AD) is often delayed due to lack ofa simple and effective screening test in primary care settings.To determine sensitivity and specificity of the validated 7 Minute Screen (7 MS) Thai version in screening elders with mild to moderate AD.The original 7 MS was...
The WW domain, first described by Bork and Sudol [1], also referred to as the WW domain containing protein (WWP) domain [2] or the extragenic suppressor of spt3 (Rsp5) repeat [3], is a recently described protein interaction module [4], [5]. The domain is approximately 40 amino acid...
Protein modification is an extremely important post-translational regulation that adjusts the physical and chemical properties, conformation, stability and activity of a protein; thus altering protein function. Due to the high throughput of mass spectrometry (MS)-based methods in identifying site-specifi...
Similar observations have been reported with certain anti-DQ monoclonal antibodies failed to recognize the DQ proteins expressed on either mouse L cell or human HeLa cell transfectants most likely due to the lack of appropriate peptides bound to the binding groove of the DQ protein16. Patient ...
Proteases are among the largest protein families and critical regulators of biochemical processes like apoptosis and blood coagulation. Knowledge of proteases has been expanded by the development of proteomic approaches, however, technology for multiplex