The mechanism of TA-mediated effects on Sp proteins was due to activation of caspases. These results now extend the number of NSAIDs that may have clinical potential for colon cancer chemotherapy and show that the anticancer activity of TA is due, in part, to targeting Sp transcription factors...
What type of detection can I use to assay interactions on the ProtoArray PPI Microarray? ProtoArray Human Protein Microarrays are compatible with fluorescent, chemiluminescent, radioisotopic, and other detection methods. Due to the high sensitivity, low back...
Sp1 is a ubiquitous zinc-finger protein that binds GC-rich elements (24) in the promoters of many housekeeping as well as tissue-specific genes. After the interaction with target GC boxes, Sp1 stimulates transcription initiation of TATA-less promoters through the recruitment of the TATA box ...
This finding challenges the view based on posterior Hox protein studies that low-affinity Hox–Exd sites are the rule to ensure Hox protein specificity. Results AP-2 is required for the development of maxillary structures During embryogenesis, AP-2 is expressed in the brain and in the maxillary...
Not only is there an improvement in specificity of the assay with single antigens but there is also a general increase in sensitivity (even for phenotypic antigen bead arrays), due to the greater number of particular antigen molecules per bead. Studies published by Gebel and Bray (2000) demons...
is a common way to regulate intracellular protein functions. However, the details of how SPY recognizes and glycosylates substrates are unknown. Here, we present a crystal structure ofArabidopsisSPY/GDP complex at 2.85 Å resolution. SPY adopts a head-to-tail dimer. Strikingly, the ...
3. 3. Uncouplers, monovalent ions and oligomycin all affect the phosphorylation of IDP more strongly than that of ADP. 4. 4. The nonspecific reaction is not due to the involvement of a nucleoside diphosphate kinase reaction. The ability to phosphorylate the various nucleoside diphosphates is ...
The tissue specificity distribution is represented by a bimodal distribution with two peaks at 1 and 31 corresponding to tissue-specific and HK genes (Additional file 16). Interestingly, the female reproductive organ, ovary, expressed many fewer unique genes, probably due to the fact that ovary ...
The importance of a particular residue in a protein can be due to many different factors, including structural stability, protein-protein interaction, protein-DNA/RNA interaction, ligand binding site and maintenance of protein functions. In most cases, it is difficult to assign a particular function...
(left) superimposed on thet-SNE of the cells. The cells belonging to the glutamatergic class are circled. The violin plots of the gene and isoform distributions show that the gene is not differentially expressed but the isoform is (right).d, Example of a gene with an isoform specific to ...