We describe the design, kinetic properties, and structures of engineered subtilisin proteases that degrade the active form of RAS by cleaving a conserved sequence in switch 2. RAS is a signaling protein that, when mutated, drives a third of human cancers. To generate high specificity for the RA...
Parkinson’s disease is a synucleinopathy that is characterized by motor dysfunction, death of midbrain dopaminergic neurons and accumulation of α-synuclein (α-Syn) aggregates. Evidence suggests that α-Syn aggregation can originate in peripheral tissu
Engineering proteases that cleave specific signaling proteins in active conformations would open new possibilities to study, regulate, and reprogram signaling pathways1. Our target here was the active state of the rat sarcoma (RAS) oncoprotein. RAS is a small GTPase that responds to growth factors, ...
a来蓝天设计公司上班是我的梦想 Comes the blue sky design company to go to work is my dream[translate] a网络连接器 Network coupling[translate] afactories life is than in the old days 工厂生活比在过去是[translate] a不想就这样轻易的地放掉你 Does not want to bleed off you on the such easil...
aFor east city's blue sky 为东部城市的蓝天[translate] awhy you not online 为什么您不在网上[translate] aSoup steamed buns 汤蒸的小圆面包[translate] aCrystal Key 水晶钥匙[translate] abut you are only fou me! 但您是仅fou我![translate] ...
This can explain why the region protected from the DNase I cleavage by these proteins is rather long (about 30 bp). We designated the proteins ERF1, ERF2 and ERF3 (endogenous retrovirus factors) in order of decreasing molecular masses. However, the complex might also include other protein(s...
White and blue square, glucuronic acid; 4S and 6S, sulfation on C-4 and C-6. MALDI-TOF N-glycome of SKOV3 Next, the presence of H type 1 or type 1 LacNAc on ovarian cancer cell lines was investigated. To determine the cellular glycan target, total N-glycans were analyzed on MALDI...
He raised his mournful eyes towards the deep blue sky, where the stars were floating like white lilies on the suce of a clear calm lak... 查看完整题目与答案 完形填空(共20小题;每小题1.5分,满分30分)阅读下面短文,掌握其大意,从各题所给的A、B、C、D四个选项中,选出最佳选项。There is ...
Your father said this calmly, taking my delicate bird hands in his strong ones and gazing at me with his piercing blue eyes that see right into my soul. “They don’t care. I don’t care, either. You’re the one who cares, and that’s all that matters. So go inside and find yo...
The nicked vector DNA containing the desired mutations is then transformed into XL1-Blue supercompetent cells. See, e.g. U.S. Pat. Nos. 5,789,166, 5,932,419, and 6,391,548. As an example, the chicken Kir2.2 protein may be used as a scaffold and the human Kir2.2 channel turret ...