The function u:[t 0 ,+∞)→R is said to be a regular solution if it is absolutely continuous together with its derivatives up to the order n-1, it satisfies almost everywhere on [t 0 ,+∞) the equation and sup {
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Fixed cells were spun down, fixative was removed and then cells were washed once in phosphate-buffered saline (PBS) and finally resuspended in 500 μl Thermo Fisher FxCycle PI/RNAse staining solution. After incubation in the dark for 30 min, cells were passed through a mesh filter sieve...
I have the system shown below in the picture, its a system of three differential equations. Wx, Wy, and Wz are constants that I define, and the initial conditions for theta, phi, and psi, are also constants that I define. I want to find the solution fo...
Tissues were fixed in 10% formalin followed by 70% ethanol, embedded in paraffin, and cut into 5 µm sections. Immunostaining was performed using the avidin biotin complex (ABC) method with 0.1% diaminobenzene solution used as the chromogen as previously described. Antigen retrieval treatment...
Using this equation of state, develop equations based on measurable properties for the changes in (a) specific internal energy, (b) specific enthalpy, and (c) specific entropy for an isothermal process. Solution The Berthelot equation is given in Eq. (3.46) as p(v−b)=RT−a(v−b)...
18 unique clusters (Fig.3a, b). The nuclei count for each cluster, with respect to each group was reported (Supplementary Data1). To identify differentially expressed genes (DEGs) in the setting of podocyte-specificKLF6induction with and without diabetes, we initially performed differential express...
Using this equation of state, develop equations based on measurable properties for the changes in (a) specific internal energy, (b) specific enthalpy, and (c) specific entropy for an isothermal process. Solution The Berthelot equation is given in Eq. (3.46) as p(v−b)=RT−a(v−b)...
First, the frozen filters were thawed and rinsed several times with a 2% sodium dodecyl sulfate solution and gently sonicated. Any clumped diatom material was gently broken apart with a glass stir rod. Organic (cellular) material was oxidized with potassium permanganate in 10 mL of 9 M sulfuric...
HeLa cells expressing microtubule-associated protein 1 light chain 3 beta (LC3B)–RFP, a canonical AP marker, were labeled with1and then cultured in Earle’s balanced salt solution to induce AP formation (Fig.4aand Extended Data Fig.10a; refs.29,30). After a short incubation (0–30 min...