The molar heat capacity of the ferroelectric betaine calcium chloride dihydrate (BCCD), (CH3)3NCH2COO路CaCl2路2H2O, was measured between 2 K and 300 K and the different phase transitions are investigated. The transition from normal to incommensurate phase shows a typical second-order anomaly at...
Experimental studies on cycling stable characteristics of inorganic phase change material CaCl2·6H2O-MgCl2·6H2O modified with SrCl2·6H2O and CMC The average value and standard deviation of latent heat of fusion were 120.62 J/g and 1.90 J/g respectively. Non-molten white solid sediments resulted...
water, they will always have an ample supply of maple syrup. If the same amount of CaCl2 is added to equal volumes of water and maple syrup, which will have the higher temperature: the water or the maple syrup? Assume the CaCl2 dissolves completely and that both the water and the maple ...
Briefly, 40 h after transfection, cells were cooled on ice, washed twice with ice-cold PBS þ þ (PBS with 1 mM CaCl2 and 0.5 mM MgCl2, pH 7.4) and incubated with PBS þ þ containing 1 mg ml À 1 Sulfo-NHS-SS-Biotin (Pierce) for 30 min at 4 °C. Unreacted biotin ...
For sites with sufficient DNA methylation signals, heatmaps show CTCF ChIP-seq signals cover 2-kb regions centered at each site. Differential DNA methylation level at a 300 bp region centered at each CTCF binding site was calculated and presented as a waterfall plot. Purple bars represent ...
Inflammatory macrophages in the peritoneal cavity of mice, elicited by heat-inactivated S. aureus, express CCR2 (Fig. 5a)47. To investigate the g-haemolysin-mediated targeting of CCR2, we monitored the presence of CCR2 þ inflammatory macro- phages in the peritoneal cavity of mice infected ...
Engineered heart muscles (EHMs) from RBM20-iPSC-CMs showed that not only active force generation was impaired in RBM20-EHMs but also passive stress of the tissue was decreased, suggesting a higher visco-elasticity of RBM20-EHMs. Furthermore, we observed a reduced titin (TTN) N2B-isoform ...
The gel was soaked in a solution containing 35% ethanol and 2% glycerol for 30 min and then dried with the application of heat (80 °C) and vacuum system. The gel was transferred onto the phosphorimager plate for 18 h and then developed on the Storm 860 Imager (Amersham Biosciences Corp...
washed with 5 mM CaCl2. Cells were incubated for a 3 h recovery period at 30 °C, 18 × gand finally plated on selective medium. Colony PCR (SapphireAmp Fast PCR Master Mix, TaKaRa) using a template prepared by boiling the clone in 50 µL NaOH (0.02 M) (99 °C,...
The inhibitor screening was performed in 1,536-well plate with 3,000 cells per well, using Huh-7 cells containing the HCV replicon subgenomic genotype 1b, maintained in DMEM, supplemented with (1 Â ) L-glutamine (L-Glue), 1 Â non-essential amino acids, 10% heat-inactivated fetal ...