It is notable that six domains were found that were not associated previously with ePKs in other organisms, based on searches of the Pfam database and Genbank. Examples of proteins containing an ePK domain along with novel accessory domains are shown in Figure 2. The unusual domains include th...
A tissue-specific atlas of protein–protein associations enables prioritization of candidate disease genes Diederik S. Laman Trip Marc van Oostrum Pedro Beltrao Nature Biotechnology(2025) Deciphering the tumor immune microenvironment from a multidimensional omics perspective: insight into next-generation CAR...
Palmitoylation increases hydrophobicity at specific points in protein structures that allow tethering/interac- tion of proteins to membranes, a process that can regulate protein trafficking, compartmentalization and cell sur- face expression. Classic examples of soluble proteins that associate with membranes ...
While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis....
Progression through the Caulobacter cell cycle is driven by the master regulator CtrA, an essential two-component signaling protein that regulates the expression of nearly 100 genes. CtrA is abundant throughout the cell cycle except immediately prior to DNA replication. However, the expression of CtrA...
The pCAGGS-RFP vector (gift from D. Henrique), which contains the CAGGS promoter and the cDNA of monomeric red fluorescent protein (RFP; Clontech) [27] was used to control the electroporation efficiency. Embryo electroporation and imaging Chicken embryos were explanted and electroporated at stages...
Human α-galactoside or Bacillus anthracis protective antigen are examples of biotherapeutic proteins whose solubility has been successfully redesigned by using SolubiS, reducing their aggregation propensity while maintaining their functional activity [106]. Such approximation has also been translated to the...
Other well-known examples of proteins with asymmetric localizations are Miranda, Prospero and Staufen during Drosophila neuroblast division[64] and the Par proteins during C. elegans development[65]. Besides the localization of proteins or protein complexes, RNAs are often asymmetrically stored and thus...
of an enzymatic reaction. Given the high sensitivity of radioisotope labeling, nanogram levels of GAG degradation can be detected. As examples, HSPE is assayed using recombinant Syndecan-4 as the substrate, human HYAL4 is assayed using Serglycin as the substrate, and bovine SPAM1 is assayed ...
The classic examples are MDM2 and IAPs, among others. There has been interest in developing inhibitors against MDM2 that regulate the expression levels of tumor suppressor and proapoptotic protein p53. Nutlin-3 and JNJ-26854165 are ubiquitin ligase inhibitors that are directed against MDM2 and are ...