It is notable that six domains were found that were not associated previously with ePKs in other organisms, based on searches of the Pfam database and Genbank. Examples of proteins containing an ePK domain along with novel accessory domains are shown in Figure 2. The unusual domains include th...
While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis....
Progression through the Caulobacter cell cycle is driven by the master regulator CtrA, an essential two-component signaling protein that regulates the expression of nearly 100 genes. CtrA is abundant throughout the cell cycle except immediately prior to DNA replication. However, the expression of CtrA...
In digenic inheritance, pathogenic variants in two genes must be inherited together to cause disease. Only very few examples of digenic inheritance have been described in the neuromuscular disease field. Here we show that predicted deleterious variants inSRPK3, encoding the X-linked serine/argenine ...
Palmitoylation increases hydrophobicity at specific points in protein structures that allow tethering/interac- tion of proteins to membranes, a process that can regulate protein trafficking, compartmentalization and cell sur- face expression. Classic examples of soluble proteins that associate with membranes ...
To provide evidence for public health recommendations regarding dietary modifications in choice of protein sources that may promote long-term health
The pCAGGS-RFP vector (gift from D. Henrique), which contains the CAGGS promoter and the cDNA of monomeric red fluorescent protein (RFP; Clontech) [27] was used to control the electroporation efficiency. Embryo electroporation and imaging Chicken embryos were explanted and electroporated at stages...
of an enzymatic reaction. Given the high sensitivity of radioisotope labeling, nanogram levels of GAG degradation can be detected. As examples, HSPE is assayed using recombinant Syndecan-4 as the substrate, human HYAL4 is assayed using Serglycin as the substrate, and bovine SPAM1 is assayed ...
Other important examples of lipid excess management by LDs (e.i., in adipocytes, hepatocytes and macrophages) are described in details in the following sections. LDs are also fundamental for supporting cell survival during complete nutrient deprivation as LD breakdown fuels mitochondrial β-oxidation ...
different cell types or in response to stimuli revealed that TSS selection within TSRs can be highly dynamic21,22. We therefore set out to investigate motif grammar from the perspective of each individual TSS, rather than from the perspective of open chromatin, a specific protein or epigenetic ...