The radioactivity was read using a 96 well format MicroBeta2 scintillation counter (PerkinElmer). The resulting data were normalized to binding in the presence of Vehicle (100%) or 10 μM naloxone/U50,488 (0%), and used to calculate the KI based on the previously established KD of 3H-...
The tracer uptake in the VOI at each time frame was calculated as a standardized uptake value (SUV) according to the following formula:SUV=RadioactivityVOIBqmLInjected ActivityBq×Body weightg A correction for radioactive decay and residual activity in the syringe was applied. SUV values were ...
A mixture of ""^(239)Pu and ""^(240)Pu has a specific activity of 6 xx 10^(9) dis/s/g. The half lives of the isotopes are 2.44 xx 10^(4) y and 6.08 xx 10^(3)
One aliquot of the supernatants was used for measuring the total radioactivity ([3H]-2-DG and [3H]-2-DG 6-phosphate ([3H]-2-DGP)). A second aliquot of the supernatants was treated with 1 N Ba(OH)2 and 1 N ZnSO4 to remove [3H]-2-DGP, and the [3H]-2-DG count was ...
Using SiFA-DMFP (4a) as the precursor, we found radioactivity incorporations in acetonitrile using TBAHCO3 of up to 61% in the crude reaction mixture (Table 4) and an overall RCY after purification of up to 42%. Under these conditions, the specific activity was calculated to be in the ...
(17) and had a specific activity of 3.26 × 106 cpm/ml. Parasites were harvested on fiberglass filters, and cell-incorporated radioactivity was determined by liquid scintillation spectrometry. For flow cytometry (FCM), parasites (106) were washed and labeled with biotin-conjugated peanut agglutinin...
The concentration of the unknown substrate in the body fluid is established by counting radioactivity of the aqueous phase of the sample mixture after competition with unlabeled substrate for binding with insolubilized pure receptor. CPM are compared with the aforementioned standard curve. The control ...
1. A stable conjugated compound of formula (II): wherein X═S; n is an number from 2-10; P is a protein, having antigenic activity which is bound through at least one lysine residue to (II); and m is a number ranging from 1 to the number of available lysine residues on said P...
dose of radioactivity per gram of tissue Tissue4 h24 h48 h Tumor: 125I-BsAb5.87 ± 2.294.21 ± 0.782.78 ± 0.56 131I-CE-DTPA2.53 ± 0.801.30 ± 0.361.36 ± 0.41 Liver: 125I-BsAb3.78 ± 0.840.20 ± 0.030.07 ± 0.04 131I-CE-DTPA2.92 ± 0.620.29 ± 0.050.14 ± 0.05 ...
. When the peptides are prepared for later use within the bsAb system, they are advantageously cleaved from the resins to generate the corresponding C-terminal amides, in order to inhibit in vivo carboxypeptidase activity. The haptens of the immunogen comprise an immunogenic recognition moiety, ...