iis the fitted value at timetiwhile values ofa, b, c, k1, k2, andk3are all constrained to⩾0. Initial rate of BNA was calculated as the linear slope of the fitted time activity curve over 15 s starting at the arrival of11C-nicotine in the brain. Values of maximal concentration (...
label. One can also use a quenching ligand to reduce intensity, for example, where more than one fluorescent label has been used, to create a three dimensional structural/activity comparison of a receptor conformation. The fluorescent labelled ligand can be used directly and quantitatively to measur...
Finally, the filters were air-dried and the radioactivity remaining on the filters was determined by liquid scintillation counting using a microplate scintillation counter (Chameleon-Hidex). Alter- natively, the membranes were punched out of the filtration plate using Multiscreen Multiple Punch Kit (...
Spearman-based hierarchical clustering was used to determine compounds with the most similar patterns of activity against 102 barcoded cell lines in PRISM. Four highly related clusters of activity each demonstrate compounds with similar modes of action (from left): corticosteroids, HMG-CoA reductase in...
Cells were then harvested and radioactivity counted on a liquid scintillation counter. Human leukocyte antigen (HLA) typing was done on the PBL of the donors by Dr Jack Silver, North Shore University Hospital. CD8+ cells. T cells were purified from PBL of healthy controls by rosetting with ...
mass, size, surface area, structure, charge, radioactivity), chemical aspects (molecular composition, solubility, elemental contents) and biological features (species, microbial viability, allergens, etc.). Nevertheless, for many important particle characteristics there is still a substantial need for new...
However, after initially growing rapidly on inserts, the mitotic activity of the ES cells declines after 4-6 weeks of culture. To extend the culture the cells have to be transferred to a feeder of STO cells. TABLE 3 Establishment of cES cells from single embryos on either inserts or a ...
α particles must be monitored to be managed as radioactive diagnostic agents or nuclear activity indicators. The new generation of perovskite detectors suffer from limited energy resolution, which affects spectroscopy and imaging applications. Here, we
FIGURE 4. Methylation activity and substrate binding properties of M.EcoP15I-M357P. A, methylation activity of wild type and mutant MTases. Methylation reactions were carried out as described under “Experimental Procedures.” B, electrophoretic mobility shift analysis. Increasing concentrations of wi...
Transport was stopped and the oocytes were washed and analysed for radioactivity as described before [1], [18]. The uptake rates inhibited by phlorizin or cyanine 863 of 8–10 paired oocytes were calculated. Means±S.E.M. are presented. 3. Results 3.1. Cloning of a pRS1-homologous cDNA ...