An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progressionCell Research44.12023 Clinical and translational values of spatial transcriptomicsSignal and Transduction and Targeted Therapy38.1042022 ...
- 适用性:如果研究需要全面了解组织中的基因表达情况,并且有条件使用专为FFPE样本优化的试剂盒,Visium可以是一个合适的选项。 3.Stereo-seq: - 优点:Stereo-seq提供极高的空间分辨率,理论上非常适合复杂组织结构的细致研究。 - 适用性:尽管Stereo-seq技术前沿,但对样本的RNA质量要求可能很高,而FFPE样本中的RNA通常...
今年3月 Nature子刊《Nature Methods》发表了一篇关于空间转录组学的全面综述:Museum of spatial transcriptomics,作者对1987年以来有关空间转录组学的文献进行了整理回顾,并对该领域的趋势进行了深入分析,如…
今年3月Nature子刊《Nature Methods》发表了一篇关于空间转录组学的全面综述:Museum of spatial transcriptomics,作者对1987年以来有关空间转录组学的文献进行了整理回顾,并对该领域的趋势进行了深入分析,如实验技术的使用、研究的物种/组织和使用的计算方法等。 技术背景-Prequel era 一些重要的空间转录组学技术可以追溯...
Spatial transcriptomics provides new insights into the molecular mechanisms underlying organ and embryonic development and human pathological tissues.aStereo-seq maps spatiotemporal transcriptome dynamics in developing mouse embryos, detailing tissue-specific identities at different stages.bUsing scRNA-seq and ST...
腾讯AI Lab联合深圳华大生命科学研究院团队,开发了一种基于自监督学习的空间转录组(spatially resolved transcriptomics,SRT)细胞注释方法Spatial-ID(SPATIAL cell type IDentifification),它集成了迁移学习和空间嵌入策略。该方法通过迁移学习从已有的单细胞转录组数据集迁移单细胞表达谱知识。该方法通过嵌入空间信息,利用细...
Although bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) have provided insights into cell types and heterogeneity in the respiratory system, the relevant specific spatial localization and cellular interactions have not been clearly elucidated. Spatial transcriptomics (ST) has filled this ...
High-definition spatial transcriptomics (HDST), a method similar to Slide-seq in its use of a barcoded bead array, allows for higher resolution by using beads that are smaller than single cells. The barcoded beads are enriched with poly(dT) nucleotides and deposited into 2μm wells to obta...
腾讯AI Lab 联合深圳华大生命科学研究院团队,开发了一种基于自监督学习的空间转录组(spatially resolved transcriptomics,SRT)细胞注释方法Spatial-ID(SPATIAL cell type IDentifification),它集成了迁移学习和空间嵌入策略。 该方法通过迁移学习从已有的单细胞转录组数据集迁移单细胞表达谱知识。该方法通过嵌入空间信息,利用...
Here we show the use of spatial transcriptomics to analyse a fully intact Carnegie stage 7 human embryo at single-cell resolution, along with immunofluorescence validations in a second embryo. Employing 82 serial cryosections and Stereo-seq technology, we reconstructed a three-dimensional model of ...