Quantification of miRNA expression Total RNA, containing small RNA, was isolated from the whole splenocytes using miRNeasy Mini Kit (Qiagen, Valencia, CA, USA). On-column DNase digestion with RNase-free DNase (Qiagen) was performed to remove residual amounts of DNA contamination in the ...
Data analysis Statistical analyses were performed with SPSS for Win- dows (release 13.0 standard version, SPSS Inc., Chicago, Illinois, USA). A chi-square test was used to compare in- fection rates between age groups. Differences were con- sidered statistically significant when P < 0.05. ...
Moreover, the intracellular lipids of microalgal cells were also visualized via Nile red (Sigma-Aldrich, USA) staining. 3 mL of the cultures was taken and centrifuged at 3800 rpm for 20 min, and the pellet was re-suspended in 0.5 mL phosphate buffer saline solution (0.1 M)...
ME, USA). All mice were housed in the phase IV animal facility of Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech. All procedures were approved by the Virginia Tech Institutional Animal Care and Use Committee (IACUC). The male and female NZB/WF1mice...
The location of each trap within the 43 block area of the DTES, and the number of rats caught in each trap that were infected with the nematode or had associated stomach pathology, and rats with neither the nematode nor stomach pathology were mapped using ArcGIS 10.0 (ESRI, Redlands, USA)...
RNA was extracted from PC12 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). For cDNA synthesis, the extracted RNA was reverse transcribed using a SuperScript III First-Strand Synthesis System (Invitrogen). Quantitative real-time polymerase chain reaction (PCR) was performed using SYBR ...
Jan P. Kraus (Department of Pediatrics, Univer- sity of Colorado School of Medicine, USA) for critical reading of the manuscript. References Barnes, W. M. (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bac- teriophage templates. Proc Natl Acad...
(SEM; JEOL JSM-6500F, JEOL-USA, Peabody, MA, USA), and transmission electron microscopy (TEM; LEO 912AB, LEO, Oberkochen, Germany). Samples were critical-point-dried (Polaron E3000 Series II, Thermo Scientific, Milan, Italy) for SEM or acid cleaned with 1:1:4, sample: HNO3: H2SO4...
The birds were probably infected before importation to Czech Republic, since they were in quarantine and then died. However, the presence of great turacos in zoos and private breeding facilities and their potential escape or release to the wild should be monitored, since these birds could spread...
Anti-annexin 2 (Novus Biological, Colorado, CO, USA), anti-vimentin (Santa Cruz Biotechnology, Dallas, TX, USA), anti-SCARB-2 (R&D System, Minneapolis, MN, USA), anti-PSGL-1 (Santa Cruz Biotechnology, Dallas, TX, USA), anti-heparan sulfate (Merck, Rahway, NJ, USA), anti-galectin...