The trypsin inhibitor crystallizes in a monoclinic unit cell of symmetry P2(1) and dimensions a = 25.919(7) A, b = 43.23(1) A, c = 19.905(5) A, and beta = 103.63(2) degrees. The assymmetric unit contains 1 molecule of molecular weight 6800. The crystal, which has been found to...
Molecular weight determinations and activity measurements indicate that SBTIA 1 and A 2 form trypsin-trypsin inhibitor complexes in the ratio of 1:1. Commercial preparations of 5X crystallized soybean trypsin inhibitor preparations frequently contain 5–50% impurities. A Chromatographic method was ...
Trypsin inhibitor is the primary antinutritional factor in soybean meal (Araba and Dale, 1990a,b; Anderson-Hafermann et al., 1992; Mian and Garlich et al., 1995), which is a globulin-type protein having a molecular weight of 24,000 and isoelectric point of 4.5 (Kunitz, 1945). Trypsin...
The Kunitz-type trypsin inhibitor from soybean (STI) consists of 181 amino acid residues with two disulfide bridges. Its crystal structures have been deter... HK Song,SW Suh 被引量: 13发表: 1998年 Separation and Some Properties of High Molecular Weight Inhibitor from Commercial Soybean Trypsin ...
Kunitz trypsin inhibitor trypsin inhibitors yes 91/6 patients; 5712/14 patients; 621/5 patients; 656/45 patients IgE binding, inhalation testing (uncontrolled) adults, children 62DBPCFC and SPT to soybean; 63possible anaphylaxis linked to KTI; 64symptoms after inhalation (uncontrolled) 9, 57, 62...
Product PDI,% Trypsin inhibitor activity, mg/g Pectinsmg/g Antigens, mg/g Soybean flour non toasted 90 23.9 7.3 610 Soybean flour slightly toasted 70 19.8 4.5 570 Soybean flour toasted 20 3.1 0.05 125 Concentrate of soybean extracted 6 2.5 <0.0001 <0.02 Table 14. Concentration of anti-nut...
They verify that Chicks fed diets containing any of the raw soybean varieties gained less weight than did chicks and among the raw soybean treatments, there was a greater effect on growth performance by removing both lectins and Kunitz trypsin inhibitor (LFKF), than by removing each anti...
Werner MH, Wemmer DE (1992) Three-dimensional structure of soybean trypsin/chymotrypsin Bowman-Birk inhibitor in solution. Biochemistry 31(4):999–1010 Article CAS PubMed Google Scholar Whelton SP, Hyre AD, Pedersen B, Yi Y, Whelton PK, He J (2005) Effect of dietary fiber intake on blo...
Cells were grown under conditions as described above, harvested by trypsinization and centrifugation at 1500 × g for 5 min, washed with PBS and fixed with 80% ethanol. Cells were then washed with PBS, resuspended, stained by adding propidium iodide (at a final concentration of 50 mg/L) an...
Generally speaking, soybean contains about 40% protein, and 20% oil with trace amounts of phospholipids, vitamins, minerals, trypsin inhibitors, phytates, oligosaccharides, and isoflavones (Liu, 1997). The numerous uses of this crop may be attributed to its unique amino acid and fatty acid ...