in a hybridization solution containing 25 mM KPO4 (pH 7.4), 5× SSC, 5× Denhart's solution, 50 μg/mL denatured, sonicated salmon sperm DNA, 50% formamide, 10% Dextran sulfate, and 1-15 ng/mL probe (about 5×107 cpm/μg), and wash steps at about 50° C. with a wash ...