Sampling of husk in the three main macadamia producing provinces in South Africa, namely Limpopo, Mpumalanga and KwaZulu Natal, was conducted from November to March of 2019/2020 and 2020/2021. As husk rot is often sporadic, structured sampling was not possible across the three provinces. Therefor...
(2) drank heavily during a previous pregnancy; (3) had eight or more drinks per week during the current pregnancy or had one binge of three or more drinks any day of the week; or (4) had a score of 8 or more on the AUDIT or a score of 2 or more on the SAQ, which is ...
3.4. Antiproliferative Assays Non-small cells of human lung cancer cells (A549; ATCC: CCL 195) and African Green monkey kidney fibroblasts (Vero; ATCC: CCL81) were grown in Eagle's minimum essential medium (MEM; Cultilab, Campinas, Brazil) supplemented with 1% glutamine (Gibco, Carlsbad, CA...
2.3. Sample Collection and Laboratory Procedures Blood was collected by venipuncture into two 8.5 mL serum separator tubes by a South African Nursing Council–registered nurse. Following centrifugation, serum was refrigerated (4 ◦C) until delivery to the National Institute for Communicable Diseases ...
Selected compounds were searched against the built- in database in the MS-FINDER system: NANPDB (Northern African Natural Products Database), KNApSAcK, COCONUT, T3DB (the toxin and toxin target database), and NPA (Natural Products Atlas), and only structures with a score above 5 were ...