TMB (3', 3', 5', 5', -四甲基联苯胺)是辣根过氧化物酶(HRP)的显色底物,是目前HRP系统ELISA实验的常用显色试剂。TMB显色底物工作液在HRP的催化下,形成蓝色的阳离子产物,在370 nm和652 nm处有主要吸收峰。加酸终止反应后,蓝色转变为黄色,可以在450 nm波长下测定。本试剂盒使用方便,灵敏度高,效果稳定,...
Soluble TMB Kit(20×) 产品货号:CW0050S 产品规格:100ml TMB底物显色试剂盒 TMB (3', 3', 5', 5', -四甲基联苯胺)是辣根过氧化物酶(HRP)的显色底物,是目前HRP系统ELISA实验的常用显色试剂。TMB显色底物工作液在HRP的催化下,形成蓝色的阳离子产物,在370 nm和652 nm处有主要吸收峰。加酸终止反应后...
All plates were developed with 50 µL/well 3,3′,5,5′-Tetramethylbenzidine (TMB; ThermoFisher, Waltham, MA), stopped by the addition of 50 µL/well 1 M H2SO4 and read at 450 nm using a SpectraMax plate reader (Molecular Devices, Sunnyvale, CA). For dilutions of antibodies used...