Bacterial community composition was assessed using 16 S rRNA high-throughput sequencing. DNA was extracted from 1.0 g of soil using the FastDNA SPIN Kit according to the manufacturer’s instructions. The quality and quantity were measured using a NanoDrop spectrophotometer (NanoDrop Technologies, ...
To extract DNA from the soil microbial incubation experiment, a soil DNA Extraction Kit (Omega Bio-Tek D5625, Norcross, GA, USA) was employed. An ultraviolet (UV) spectrophotometer was used to evaluate the concentration and purity of the isolated DNA (Thermo NanoDrop2000, Waltham, MA, USA)....
®Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA) according to the manufacturer’s instructions. The concentration and purity of DNA were determined using a NanoDrop 2000 spectrophotometer. The DNA extraction quality was evaluated using 1% agarose gel electrophoresis. Two primers, 338F (5′-...
Total DNA extraction was performed from soil samples of 0.25 g using the PowerSoil DNA Isolation Kit (MO BIO Laboratories, Inc, Carlsbad, CA, USA) according to the manufacturer’s protocol. The fluorescent intercalating dye Sybr Green, which binds to double-stranded DNA, was used. The total ...
To find out if the strains promoted the growth of maize seedlings during the early stages, seeds were sterilized with 5% sodium hypochlorite and washed with sterile water four times to remove the sodium hypochlorite [50]. Twenty-four seeds were treated with each bacterial strain in liquid culture...
Due to the development of urbanization and industrialization, a large amount of cultivated land resources has been occupied, while new reclamation land could expand the supply of usable land for food security. Organic fertilizers, such as crop residues,
We explored the characteristics of soil bacterial communities and their ecological functions under different types of vegetation reclamation in open-pit mines on the Loess Plateau, which is the guiding significance for the selection of vegetation and the
R6825-00 OMEGA土壤RNA提取试剂盒,Soil RNA Mini Kit Omega Bio-Tek公司,DNA重组技术被广泛应用于人类、动物及植物生物学研究领域。 产品简介: 产品品牌:OMEGA 产品货号:R6825-00 产品名称:R6825-00 OMEGA土壤RNA提取试剂盒,Soil RNA Mini Kit 产品规格:kit 本产品详情页介绍里面出现*或X代表有网络不能使用的...