随后,我们进行了酶联免疫吸附测定(ELISA),发现SNHG16的过表达增强了LG处理的hRMEC中的低p65活性,而SNHG16敲低则降低了HG处理的hRMEC中的高p65活性(图3E)至于PI3K / AKT途径,在SNHG16过表达后,PI3K表达和p-AKT的水平升高,表明PI3K / AKT途径的正调节。相比之下,SNHG16敲低后PI3K表达和p-AKT降低(图3F...
SNHG16的耗竭明显提高了MTB感染巨噬细胞的miR-140-5p水平,证实miR-140-5p是SNHG16的潜在靶miRNA,并受到负调控。通过调节MTB感染的巨噬细胞中miR-140-5p水平来确认SNHG16是否参与其进展,我们在MTB感染的巨噬细胞中共转染了si-SNH...
SNHG16通过在Jurkat细胞中过滤let-7c-5p来抑制凋亡并促进增殖 SNHG16通过Sponging let-7c-5p抑制凋亡并促进细胞增殖 A)在转染阴性对照、siSNHG16或siSNHG16+let-7c-5p抑制剂后,Jurkat细胞被Annexin-V-FITC/PI染色,并通过流式细胞仪分析检测凋亡。(B)Jurkat细胞在转染阴性对照、siSNHG16或siSNHG16+let...
研究发现,长链非编码RNA(lncRNA)SNHG16 在许多不同类型的人类肿瘤里面异常高表达,并作为竞争性内源 RNA,通过结合miRNA,调控下游基因的表达促进肿瘤的发生进展。 细胞周期紊乱和上皮间质转化是肿瘤细胞的重要特征,但是SNHG16 在肿瘤细胞周期调控和上皮间质转化(EMT)中的功能和机制还没有 得到系统的研究,很大程度...
目的下调长链非编码RNA SNHG16对人胃癌细胞HGC-27细胞周期的影响。方法实时荧光定量PCR(quantitative real time-PCR,qRT-PCR)法检测SNHG16siRNA在HGC-27细胞中的干扰效率;流式细胞术(FACS)检测敲低SNHG16后对HGC-27细胞周期的影响;蛋白免疫印迹法(Western blot)检测周期相关蛋白细胞周期素依赖性蛋白激酶2(CDK2...
(lncRNA) SNHG16, which functioned as a ceRNA by sponging miR-16–5p and therefore potentiated the TGF-β1/SMAD5 pathway to upregulate CD73 levels. Our results suggest that targeting CD73+γδT1 cells or blocking tumour cell-derived exosomes (TDEs) could be a strategy for BC treatment ...
SNHG16对人胃癌细胞HGC-27细胞周期的影响.方法 实时荧光定量PCR(quantitative real time-PCR,qRT-PCR)法检测SNHG16 siRNA在HGC-27细胞中的干扰效率;流式细胞术(FACS)检测敲低SNHG16后对HGC-27细胞周期的影响;蛋白免疫印迹法(Western blot)检测周期相关蛋白细胞周期素依赖性蛋白激酶2(CDK2)的改变情况.结果 ...
SNHG16 could bind with miR-339-5p, and NLRP1 was found to be the downstream mRNA of miR-339-5p. SNHG16 silencing significantly abolished the LPS-induced upregulation of NLRP1 through miR-339-5p downregulation. The upregulation of miR-339-5p inhibited the pro-apo...
USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses ...
SNHG16 was highly expressed in glioma and may exert oncogenic function as a competing endogenous RNA (ceRNA) to regulate EGFR by sponging of miR-373-3p through activating PI3K/AKT pathway, which providing a new insight of the regulatory network of lncRNA SNHG16 in the development of glioma...