2021年6月,李琛主任医师在《FRONTIERS IN ONCOLOGY》杂志发表题名为“Long Noncoding RNA SNHG12 Promotes Gastric Cancer Proliferation by Binding to HuR and Stabilizing YWHAZ Expression Through the AKT/GSK-3β Pathway.”——长...
Feinberg及其同事们使用了一种动脉粥样硬化小鼠模型,其中这些小鼠在12周大时开始出现动脉粥样硬化病变。他们从血管壁最内层分离出RNA,并观察整个基因组所编码的所有RNA,以便寻找在疾病进展或消退过程中哪些RNA的活性发生了变化。其中活性动态变化最为明显的RNA之一是SNHG12:它是一种lncRNA,不编码蛋白,但可在人类、猪...
SNHG12Esophageal squamous cell carcinomaPrognosisCompeting endogenous networksPurposeEsophageal squamous cell cancer (ESCC) has high rates of recurrence and mortality. Small nucleolar RNA host gene 12 (SNHG12) is known to promote the progression of several cancers. Therefore, we aimed to investigate ...
长链非编码RNA SNHG12被认为与细胞的氧化还原稳态具有相关性,其能否调控过氧化氢过载引起的神经细胞氧化损伤目前仍未知。 目的:探究长链非编码RNA SNHG12在过氧化氢诱导神经细胞HT22氧化损伤中的作用及机制。 方法:①100 μmol/L过氧化氢干预HT22细胞6 h,建立细胞氧化损伤模型。②通过转染提升HT22细胞中SNHG12表达...
总的来说,研究人员发现SNHG12在TMZ耐药GBM细胞系和组织中高表达;SNHG12基因敲除可在体外恢复TMZ耐药GBM细胞的TMZ敏感性;SNHG12过表达可抵抗TMZ;DNA甲基化和SP1共同调控SNHG12的表达;SNHG12充当miR-129-5p的海绵;在促进细胞增殖和抑制细胞凋亡中发挥重要作用,从而增加MAPK1和E2F7的表达,激活MAPK-ERK通路;MAPK1和...
［摘要］ 目的：研究长链非编码RNA （LncRNA ）小核仁RNA 宿主基因12（SNHG12）调控miR -138-5p/低氧诱导因子-1α（HIF -1α）轴改善缺氧/复氧（H/R ）人血管内皮细胞损伤的作用。方法：体外培养人脐静脉内皮细胞（HUVECs ），随机分为对照组、H/R 模型组、H/R+LncRNA SNHG12过表达组、H/R+miR -...
The small nucleolar RNA host gene 12 (SNHG12) has been reported to play an important role in the tumorigenesis and progression of PCa, but the functional underlying mechanism has not been studied cle...
relieved the cardiomyocyte apoptosis induced byH/R stimulation. Additionally, the suppression of SNHG12 signif i cantly boosted the H/R-induced expression and theproduction of TNF-α, IL-6, and IL-1β, as well as the activation of NF-κB, which were fully reversed after overexpression of...
产生的细胞毒性损伤最为显著.Snhg12在OGD12h/R24h处理后表达水平达到最高.Snhg12在原代神经元的细胞核与细胞质中均大量广泛表达.Snhg12敲减后的原代神经元在OGD/R处理后,细胞毒性损伤增加:LDH释放增加死亡率上升,存活率下降,Bcl-2水平下降,Bax水平升高,Bcl-2/Bax比值下降.Snhg12敲减组原代神经元细胞在OGD/R...
目的: 探究长链非编码RNASNHG12在乙肝病毒X蛋白(HBx)诱导肝癌发生过程中的作用. 方法: 把课题组构建的肝前体细胞14-19,EGFP-14-19,HBx-EGFP-14-19通过小鼠肝门静脉注射到体内;采用qRT-PCR,Westernblot方法检测30d,90d,180d,360d小鼠肝脏组织及细胞模型中HBx,SNHG12以及下游调节基因的mRNA和蛋白表达情况;使用Si...