Of note, the signal of cBAF and PBAF was wider at TSSs than at dCREs; therefore, we cannot rule out that part of the signal occurs at promoter-proximal regulatory elements in addition to the TSS. Download: Download high-res image (1MB) Download: Download full-size image Figure 4. ...
9g), this does not rule out the possibility of direct ubiquitin engagement by the acidic C-terminal region of SSX when SS18-SSX-bound complexes are docked on nucleosomes. In a similar manner, Dot1L will not bind free ubiquitin but will be poised to interact with H2BK120Ub during substrate...
Samples were diluted to a final concentration of 2 M urea and digested with LysC at room temperature overnight, and then diluted further to 1 M urea and digested with Trypsin at 37 °C overnight (for each enzyme a ratio of 1:125 enzyme:protein was used). Samples were labeled usi...
To rule out the possibility that the signals were due to cross-reaction of the anti-SYT serum with some of the enriched SNF/SWI components, we performed a mass spectrometric analysis of tryptic peptides obtained from several SDS-polyacrylamide gel slices in the 53–55 kDa range (Fig. 1). ...
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We cannot rule out the possibility that these or other complexes present in the Drosophila extracts are contributing to the transcription we observe. However, since purified E-RC1 is absolutely required for transcriptional activation and chromatin disruption, these complexes are not sufficient to ...