PCR products were analysed by horizontal electrophoresis in a 2% agarose gel in TBE buffer (Tris-Borate 0.045M; EDTA 1 mM) and a 100bp (GeneRulerTM 100 pb DNA Ladder) ladder as a ruler. The gel was stained with ethidium bromide and observed under an UV transiluminator at 365 nm. For ...
Because the ossuary is situated about six meters above the beach on top of an hourglass-shaped limestone outcrop, we had to construct and scale a rough pole ladder to reach our goal. Henry went up first into the tangle of palm trees and bushes that, from a distance, gives this top-...