PCR products were analysed by horizontal electrophoresis in a 2% agarose gel in TBE buffer (Tris-Borate 0.045M; EDTA 1 mM) and a 100bp (GeneRulerTM 100 pb DNA Ladder) ladder as a ruler. The gel was stained with ethidium bromide and observed under an UV transiluminator at 365 nm. For ...