1:2,000), Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) (Sigma-Aldrich, #A8592; 1:2,000), anti-HA (Abm, #G036; 1:2,000), anti-HA High Affinity (Roche, #11867423001; 1:1,000), anti-Histone H3 (Cell Signaling, #4499; 1:1,000), anti-α-tubulin (Sigma-Aldrich, #T6199; 1...
Cells were harvested and lysed in lysis buffer (50 mM Tris at pH 8.0, 500 mM NaCl, 0.5% Nonidet P-40, 1 mM dithiothreitol and protease inhibitors). The protein extracts were then immunoprecipitated with antibody or control serum (Santa Cruz Biotechnology) at 4 °C for 4 h. ...
Vimentin Antibody (AF7013; Affinity), Beta Actin Monoclonal antibody (66009-1-Ig; Proteintech), NF-κB p65 (D14E12) XP® Rabbit mAb (8242T; CellSignalingTechnology), Phospho–NF–κB p65 (Ser536) (93H1) Rabbit mAb (3033T; CellSignalingTechnology), GAPDH Antibody (AF7021; Affinity). ...
Antibodies against Snail (3,895 s, Cell Signalling, mouse monoclonal, L70G2, 1:2,000), Slug (9585S, Cell Signalling, rabbit, C19G7, 1:1,000), PFKP (sc130227, Santa Cruz, rabbit polyclonal, C-23, 1:1,000), PFKM (HPA002117, Sigma, rabbit polyclonal, 1:1,000), PFKL (sc...
Galiximab (anti-CD80 monoclonal antibody) is a primatized (human IgG1 constant regions and cynomologus macaque variable regions) monoclonal antibody that is currently in clinical trials. Galiximab inhibits tumor cell proliferation through possibly cell signaling-mediated effects. Thus, we hypothesized...
Mouse monoclonal anti-human E-cadherin antibody and rat tail collagen were from BD Transduction Laboratories, Lexington, KY. Mouse monoclonal anti-human vimentin antibody and mouse monoclonal anti-human Stat3 was from Santa Cruz Biotechnology, Santa Cruz, CA. Rabbit monoclonal anti-phospho-Stat3 were...
Decreased levels of interleukins (TNF-卤, IL-1卤, CINC-1, IL-17, MMP-1, Cathepsin K) on synoviocyte cells also confirmed reduced osteoarthritis after treatment with VB-P4 (40 g/mL). Flow cytometric analysis of monoclonal antibody (CD3, CD4, and CD8) exhibited increased expression on ...
1,† , Ewa Macierzynska-Piotrowska 1, Wanda Klopocka 2 , Patrycja Przygodzka 3 , Magdalena Karakula 1, Karolina Pestka 1, Marta E. Wawro 1and Jolanta Niewiarowska 1,* 1 Department of Molecular Cell Mechanisms, Medical University of Lodz, 92-215 Lodz, Poland ...
probed overnight at 4 °C under gentle shaking with the following primary antibodies: anti-phosphop44/42 MAPK (p-Erk1/2) rabbit monoclonal (1:1000), anti-p44/42 MAPK (Erk1/2) rabbit polyclonal (1:1000), anti-COX-2 rabbit monoclonal (1:1000), (all purchased from Cell Signaling ...
Mouse monoclonal anti-human E-cadherin antibody and rat tail collagen were from BD Transduction Laboratories, Lexington, KY. Mouse monoclonal anti-human vimentin antibody and mouse monoclonal anti-human Stat3 was from Santa Cruz Biotechnology, Santa Cruz, CA. Rabbit monoclonal anti-phospho-Stat3 were...