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SMRT Portal环化及纠点工作 1、 目的:环状基因组环化,复制起始位点调整。 1. SMRT Portal拼接基因组(HGAP_Assembly.3),如果拼接结果很好,可以进行环化工作,则下载polished assembly fasta序列。 2. Pac Bio拼接的基因组序列两端一般有重复片段,通过环化和复制起始位点调整达到去掉其中一端的重复片段的效果。
If you are an Electrical Distributor and you need a portal password, you can call your local agent, or call or email SMRT at sales@installsmrtcontrols.com Thank you for stopping by! Now, on to my message to our Electrical Distributor Friends... In starting this new venture as President ...
SMRT_GROUP=smrtanalysis 修改完之后,记得source /etc/bashrc 5) 安装 bash smrtanalysis_2.3.0.140936.run --rootdir $SMRT_ROOT 在安装过程中,会提示一系列需要修改的信息,比如IP地址, 端口号等,对应的选择就好 6) 启动服务 $SMRT_ROOT/admin/bin/smrtportald-initd start $SMRT_ROOT/admin/bin/kodosd star...
At the end of the run, a sequencing report was gener- ated for every cell, via the SMRT portal. Thereby, the adapter dimer contamination, the sample loading effi- ciency, the obtained average read-length and the number of filtered sub-reads have been assessed. Sequence analysis Processing ...
PB_jobid – The ID number assigned to each job when the data is demultiplexed on SMRT portal. data_path – Path to where the demultiplexed data for each job is located. forward_barcode – Name of the forward primer used for the samples in the respective job, as given in the PRIMERS_...
35kAccesses 3000Citations 139Altmetric Metrics Abstract We present a hierarchical genome-assembly process (HGAP) for high-qualityde novomicrobial genome assemblies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT) DNA sequencing. Our method use...
Abstract Global yields of potato and tomato crops have fallen owing to potato late blight disease, which is caused byPhytophthora infestans. Although most commercial potato varieties are susceptible to blight, many wild potato relatives show variation for resistance and are therefore a potential source...
许多PacBio特定工具和pipelines(包括多路分解,创建CCS reads,长扩增子分析,重头组装和表观遗传分析)中可用PacBio SMRT分析套件(开源的,www.pacb.com/support/softwaredownloads/),通过命令行或其SMRT Portal和SMRT链接图形用户界面进行分析。 SMRT在肿瘤研究中的应用...
The modified bases and motifs in the other strains were identified using the same SMRT Portal program, but with the ∆RM analysis results used as a control. All analyses used the H. volcanii DS2 genome as the reference sequence [47]. 2.5. Bioinformatics Analysis Homologs of the putative RM...