In the present study, we have evaluated the in vitro co-culture of epithelial cells and smooth muscle cells on the aligned nanofibrous scaffold made of PHBV, PHBV-gelatin, PCL and PCL-gelatin developed through electrospinning using rotating drum collector. Epithelial cells were labeled with cell ...
Cell & Tissue ResearchPalmberg L, Thyberg J. Uterine smooth muscle cells in primary culture. Cell Tissue Res 1986;246:253-62.Palmberg L, Thyberg J 1986 Uterine smooth muscle cells in primary culture. Alterations in fine structure, cytoskeletal organization and growth character- istics. Cell ...
to plaque rupture. The role of smooth muscle cell-derived macrophage-like cells in atherosclerosis was under controversial. Here, we find that SMC-derived macrophage-like cells could revert to smooth muscle cells in fibrous cap of advanced plaques, suggesting that these cells may play a protective...
In order to achieve this goal, we isolated primary SMC from small intestine and colon of smMHC Cre-eGFP/+ mice, which labeled all differentiated SMC. By using deep DNA sequencing technology, we obtained the transcriptome of SMC. The transcriptome revealed thousands of new isoforms that were ...
Figure 1. Morphological and ultrastructural features of airway smooth muscle showing (a) peribronchial view of α-actin-labeled bundles in developing airways and (b) general orientation of contractile filaments and dense bodies (arrowheads) along the long axes of individual cells. Intercellular coupling...
RNA in situ hybridization (ISH) techniques allow the examination of gene expression at the individual cell level in a histocytomorphological context. These techniques often involved the use of radioactive-labeled probes. However, because of resolution drawbacks limiting their applicability in the study...
FIG. 2 contains photographs of the immunofluorescence of MNC, SOC, and EOC labeled with antibodies to αSMA, smooth muscle MHC, and calponin. The secondary antibodies in each case were conjugated to Alexa-fluor. Cell nuclei were counterstained with Hoechst stain (blue). hVSMCs were used as ...
The labeled U937 cells (1 × 105 cells/well) were added to the confluent HASMCs and incubated at 37 °C for 1 h. The unbound U937 cells were gently removed by washing three times with PBS. The adherent U937 cells were detected using ZOE Fluorescent Cell Imager (Bio-Rad) and ...
The cells were labeled with Alpha-Smooth Muscle Actin Monoclonal Antibody (4A4) (Product # MA5-15871) at 1:200 dilution ... View More 产品信息 应用 建议稀释比 已发表文章 免疫印迹 (WB) 1:500-1:2,000 - 免疫组化 (IHC) - 查看1篇已发表文章 免疫组化(石蜡) (IHC (P...
all labeled cells were a single Rb color (Fig.2a–c, Supplementary Fig.4aand Supplementary Table3), and in one plaque (at 16 weeks), 608 of the 613 labeled cells were Cerulean+and the remaining 5 cells were mCherry+(Supplementary Table3). In terms of smooth muscle-derived cells, brachi...