FIGURE 1. Smooth muscle titin RT-PCR products. A, the diagram of the 28 exons comprising the 5′ end of the human titin gene (modified from Bang et al. (15)) illustrates exons included in RT-PCR products amplified from three commercially available RNA sources (human carotid artery, uterus...
a The diagram shows the construction strategy of smooth muscle cell-specific LncDACH1 conditional knockout mouse. b The diagram shows the construction strategy of adeno-associated Virus overexpressing LncDACH1 mouse. c, e HE staining of vascular tissue and morphometric analysis of the extent of vas...
An evolutionarily conserved vertebrate homologue of the Drosophila NK-3 homeodomain genebagpipe, Nkx3-1, is expressed in vascular and visceral mesoderm-derived muscle tissues and may influence smooth muscle cell differentiation. Nkx3-1 was evaluated for mediating smooth muscle γ-actin (SMGA) gene ...
We previously reported enhanced proliferation of smooth muscle cells on the combined exposure of HIV proteins and cocaine leading to the development of HIV-pulmonary arterial hypertension. Here, we attempt to comprehensively understand the interactions b
Yin Yang-1 (YY1) belongs to the GLI-Kruppel class of zinc finger proteins that can act as a transcriptional repressor or activator depending on the cellular context9. YY1 has been shown to regulate gene transcription by either binding to the promoter or as a cofactor of a variety of ...
The present invention is directed towards a method of regulating smooth muscle tone, comprising the introduction, into smooth muscle cells of a subject, of a DNA sequence encoding a
Cell Signal. 2014;26:2738–48. Article CAS PubMed Google Scholar Singh P, Zheng L. Dual regulation of myocardin expression by tumor necrosis factor-α in vascular smooth muscle cells. PLoS One. 2014;9:e112120. Article PubMed PubMed Central Google Scholar Liu Y, Sinha S, McDonald OG...
CKLF1 serves as a key and multifaceted determinant of diverse biological functions, such as chemotactic activities [7], pulmonary fibrosis [8] and the growth of cells in multiple organs including bone marrow, skeletal muscle, endothelium and chondrocytes [9]. Our previous study established a novel...
Yin Yang 1 (YY1) regulates gene transcription in a variety of biological processes. In this study, we aim to determine the role of YY1 in vascular smooth muscle cell (VSMC) phenotypic modulation both in vivo and in vitro. Here we show that vascular injur
Cells were characterized as hVSMCs by morphologic criteria and by immunostaining with smooth muscle α-actin. Cells were seeded in smooth muscle cell growth medium (PromoCell, Heidelberg, Germany) with 5% fetal calf serum (FCS) (Gibco Invitrogen, Carlsbad, CA, USA) and allowed to attach for ...