Most recently, the technical advance of using short, fluorescently labeled probes has allowed for the detection of single-mRNA molecules. Thus, quantification of RNA levels in single cells or even within subcellular regions is now possible without RNA isolation. In combination with the ...
按照下图指示输入需要检测的目标序列及相关参数,可以直接输入基因CDS区域序列。所有必填项输入完毕后点击“design probes” 3 软件设计结果示例如下,其特异性可在Pubmed进行比对,如果确认探针数量及位置没问题,请点击“order”。 4 根据实验室荧光...
临床上用于FISH检测的探针大致分为四种类型:染色体计数(着丝粒)探针(centromere-enumeration probes,CEP),位点特异性识别探针(locus-specific identifier probes,LSI),染色体涂染(whole-chromosome paint,WCP)探针以及光谱核型(spectral karyo...
We present a new method to detect epididymal sperm aneuploidy (ESA) in mice using simultaneous fluorescence in situ hybridization (FISH) with DNA probes sp... X Lowe,S O’Hogan,DM Ii.,... - 《Chromosoma》 被引量: 86发表: 1996年 [Preimplantation development of mouse embryos with monosomy ...
HT-smFISH uses RNA probes transcribed in vitro from a large pool of unlabeled oligonucleotides. This allows the generation of individual probes for many RNA species, replacing commercial DNA probe sets. HT-smFISH thus reduces costs per targeted RNA compared with many smFISH methods and is easily...
This approach enables accurate quantification of mRNA numbers and localization at a single-cell level. To ensure reliable results using smFISH, it is critical to use fluorescent probes that are highly specific to their RNA target. To facilitate probe design, we have created anglerFISH, a user-...
FISH was used to assess gene copy number (GCN) (MET and CEP7 probes from Vysis). Using different scoring methods, correlation with clinico-pathologic and molecular features (KRAS, EGFR) and between Met expression and GCN was investigated. Heterogeneity of Met status between different tumor cores...
细胞遗传学的检测分析可以帮助我们了解染色体的变异,进而对相关疾病更深入的了解。染色体的异常可能导致基因表达的失调或产生新的突变蛋白,从而构成癌症、不孕症和各种先天性疾病(如唐氏综合征)。非整倍体、缺失、重复和重排等都属于染色体异...
目前临床上用于FISH检测的探针的荧光素大都是绿色的和橙红色标记,可大致分为:染色体计数(着丝粒)探针(centromere-enumeration probes,CEP),位点特异性识别探针(locus-specific identifier probes,LSI),染色体涂染(paint,WCP)探针。其中CEP和LSI探针中的计数探针、融合...
The technique employs multiple single-stranded short DNA probes fluorescently labeled with organic dyes that hybridize to target RNAs in fixed cells, allowing quantification and localization of RNAs at the single-cell level and at single-molecule resolution. The protocol described here has been ...