PCR amplification of HPV DNA by three consensus primer sets and HPV genotyping by amplicon sequencing. a Representative gel image of PCR amplicon detection by high-resolution capillary gel electrophoresis. Representative samples #285 (LSIL) and #179 (HSIL) reveal MY09/11, FAP59/64, and GP-E6/...
What is the significance of a coating of cutin on the epidermis? What is the purpose of triage? How do you make it effective? What are the probable reasons for getting a smudged effect when you run agarose gel after PCR? Explain the significance of crystal violet in Gram staining. What ...
The expression of hsa-miR-194-5p and hsa-miR-195-5p was analyzed by real-time quantitative PCR, using specific stem- loop primers and U6 snRNA as the internal reference gene. Clinicopathological features were associated with miRNA expression levels. Furthermore, functional ...
Why is it necessary to purify PCR products? Why are saprophytic bacteria helpful and necessary? Why is agar a useful substance in the microbiology lab? Why is epithelial tissue important? Why is heat necessary in spore staining? Why is microbial antagonism important?
PCR products of cervical smear were analyzed for HPV by agarose gel electrophoresis. (A) PCR analysis for the presence of any HPV type; (B) PCR analysis for HPV16 or 18. The designations in the gel represent: M; molecular weight markers, N; negative control, P; positive control, 1-8;...
The PCR conditions were initial denaturation at 95°C for 3 minutes, then 40 cycles at 95°C for 5 seconds, and 60°C for 10 seconds on an Agilent AriaMx. Viral copy numbers were converted into an equivalent DNA load: 1 ng viral DNA = 1.2 × 108 copies (http://cels.uri.edu/gsc/...
PCR may not confirm T. gondii due to loss of some or all the gene during extraction, and genomic DNA of host and parasite may be insufficient to amplify on agarose gel. This may account for our lower incidence by PCR. In this case we must assume that the smear method was probably ...
The expression of hsa-miR-194-5p and hsa-miR-195-5p was analyzed by real-time quantitative PCR, using specific stem-loop primers and U6 snRNA as the internal reference gene. Clinicopathological features were associated with miRNA expression levels. Furthermore, functional enrichment analysis was ...
After clinical examination & clinical categorization two skin smpars were taken, one for Z-N staining for AFB & another for M leprae RLEP PCR. After DNA extraction & amplification, electrophoresis was done on 2% agarose gel. Presence of 129bp fragment amplicon (RLEP of M leprae) was ...
What are the probable reasons for getting a smudged effect when you run agarose gel after PCR? How is Acid-Fast staining done? Summarize the Gram stain procedure. How is Gram staining done? What is the conclusion of the gram staining technique?