3b) with a line Iloop = kt + c, where c compensates for the initial DNA amount before loop extrusion. Time traces of loop-extrusion rates were then obtained using \(k\left(t\right)=\left[{I}_{{\rm{loop}}}\left(t+dt\right)-\,{I}_{{\rm{loop}}}\left(t-dt\right)...
Colorimetric ATPase activity of wild-type and mutant SMCHD1(24–580) constructs was determined using the PiColorLock Gold Kit (Innova Biosciences) which detects the amount of free Pi bound to malachite green dye. Each reaction mixture (50 μl) contained 20 mM HEPES, pH 7.5, 1 mM MgC...