1D). In one section of a single tumor nodule, three selected regions exhibited distinct histologic features (Fig. 1E), indicating strong ITH in CRISPR/Cas9-induced liver cancer in mice. Fig. 1 Induction of primary mouse liver cancer by CRISPR/Cas9-mediated somatic mutagenesis in mice. A 34...
After treatment, mice were sacrificed, and the glioblastoma and peripheral tissues were dissected and frozen sectioned. A Leica TCS SP8 MP confocal microscopy was applied to detect the red fluorescent signal in the frozen sections. Evaluation of the therapeutic effects of the brain-directed genetic ...
1B); therefore, it is not surprising that both of these non-coding RNAs interact with some of the same cellular proteins (Section 3). Potential analogues to BC200 have also been found outside of mammals in Caenorhabditis elegans (C. elegans), although the C. elegans non-coding ...
Human papillomavirus DNA was found in 69% of squamous cell carcinomas with 30% containing high-risk HPV types. No significant viral DNA was detected in non-neoplastic lung. Patients with tumours containing viral DNA experienced improved long-term survival compared with patients with viral DNA-...
elements similar to plant small RNA systems, such as the RNAi machinery, a high percentage of short interfering RNAs originating from protein-coding and repetitive regions of the genome, and putative binding sites of the small RNAs occurring primarily in the coding section of the predicted targets...
>F.Previous genetic studies located the sex locus on chromosome 2. We used DNA polymorphisms of geographically diverseV. viniferagenotypes to confirm the position of this locus, to characterise the genetic diversity and traces of selection in candidate genes, and to explore the origin of ...
The pi and crasiRNA pools were annotated by first mapping the pools to the Meug_2.0 tammar genome assembly as described in the small RNA mapping section. Next, database predicted and de novo repeats were mapped to the genome using RepeatMasker. A small RNA was considered overlapping, or asso...
Section snippets Materials Chitosan with molecule weight (Mw) of 158 kDa and degree of deacetylation (DD) of 80% was degraded and acetylated from raw CS (Mw of 419 kDa and DD of 90%; Golden-Shell Biochemical Company, Ltd., Zhejiang, China) in our laboratory. siRNA and fluorescence-labeled...
The decline of nicotinamide adenine dinucleotide (NAD) occurs in a variety of human pathologies including neurodegeneration. NAD-boosting agents can provide neuroprotective benefits. Here, we report the discovery and development of a class of potent acti
Prior to this study, we performed a search of viral genomes for sequences similar to CRISPR spacers (see 'Previous BLASTn search' in the Methods section), which should relate viruses and their hosts. For RefSeq viruses their hosts were already described, but as previously noticed22there were...