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The following reaction mix was used for the first round PCR: 1X PCR buffer (Life Technologies), 1.5 mM MgCl2 (Life Technologies), 0.5 mM dNTPs (Thermo Scientific, Waltham, MA USA) 1 μM Alu forward primer, 6 μM Gag reverse primer, 2.5 U of Platinium Taq polymerase (Life Technologies...
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