高通量测序技术省去了烦琐的Small RNA克隆文库构建过程,可以一次性生成上百万条Small RNA序列,能够快速鉴定特定条件下表达的已知Small RNA并发现新的Small RNA,同时还可以研究不同条件下Small RNA的表达差异,并可以联合转录组测序表达谱数据进行关联分析。 A workflow for Small RNA SequencingJai PM et al., RNA Ma...
1.总RNA提取及质控:RNA总量 > 1 μg ;RIN> 6.0 2.小RNA富集:切胶范围10-50bp 3.小RNA质控:Agilent 2200质控 4.文库构建:文库分子18-30bp 5. 上机测序:Small RNA测序NovaSeq、HiSeq、Ion Proton等测序仪都可以完成。测序数据量40M reads。
Buschmann D., Haberberger A, Kirchner B,et al. Toward reliable biomarker signatures in the age of liquid biopsies-how to standardize the small RNA-Seq workflow[J]. Nucleic acids research, 2016, 44(13): 5995-6018. Miao X, Luo Q, Zhao H,et al. Genome-wide analysis of miRNAs in the ...
One of the challenges in deep sequencing of the small RNAs (small RNASeq) is in the sample preparation workflow. The small RNA sample preparation workflow involves the ligation of fixed sequences, called adapters, onto the 5' and 3' ends of the starting RNA library. This ligation step can...
Workflow Arraystar offers a complete microRNA-seq service from RNA preparation and QC, library construction, sequencing, to comprehensive data analyses. Benefits Technological Performance • Highly optimized procedures to maximize the mappable reads and deliver precise quantification ...
Small RNA-Seq Library Prep Kit Gel-free user-friendly workflow Multiplexing of up to 96 samples Ready-to-sequence libraries in less than 5 hours Wide input range from 50 pg to 1,000 ng RNA Optimized for low RNA content samples such as plasma, serum, and urine ...
The SMARTer smRNA-Seq Kit for Illumina is designed to generate high-quality small RNA-seq libraries from 1 ng–2 µg of total RNA or enriched small RNA.
nf-core/smrnaseqis a bioinformatics best-practice analysis pipeline for Small RNA-Seq. The pipeline is built usingNextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results high...
所提供的单独批次均通过额外功能验证。单个试剂通过标准酶活性和质控分析,并同时满足各单独组分页面列出的严格的附加质控标准产品类别: Small RNA Library Preparation, Infectious Diseases & ARTIC Sequencing, NEBNext® Multiplex Oligos (Adaptors & Primers), |More +应用: RNA-seq ...
Illumina TruSeq Small RNA Set C MiniSeq Kit (1 library prep and 2 MiniSeq reagents)/20005615/1 Ea 产品编号: 20005615 美 元 价: $3200.00 品牌: Illumina 产地: 美国 公司: Illumina, Inc. Product Highlights: TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA librari...