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33(Raw vs. Processed, respectively, Fig.1b). We determined the Z′-factor of the assay to be 0.52 (Fig.1c). Z′-factors range from − ∞ to 1, with the
analysis), large (>20 µm) and small (<20 µm) cells were collected in duplicates for each treatment by successive filtration of 2 L on 20 µm pore size nylon filter (Millipore, USA) and 0.65 µm pore size PVDF filter (Durapore, Germany) and stored at −80 ...
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Five-micrometer slices of non-tumor and tumor FFPE tissues were stained with hematoxylin and eosin (HE) and analyzed under a microscope (Leica DM4000, Germany). Tumor tissues were evaluated for expression of alpha-fetoprotein (AFP), cytokeratin 19 (CK19), and Golgi glycoprotein 73 (GP73) by...
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All reads were mapped to the reference genome of Mesocricetus auratus using HISAT2 v.2.2.1 as instructed (Kim et al., 2019) RNA counts were generated from Feature Counts (v.2.0.0) (Liao et al., 2014) Differential expression analysis was implemented using edgeR package (v.3.34.1) as ...
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