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This can also be used as a tool for radiation dosimetry, as it could be used to estimate the proportion of irradiated cells nuclei not traversed by a single alpha particle. Gonon et al. investigated the induction of γ-H2AX foci in HUVEC cells irradiated with alpha particles through the ...
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At the same flow rate and time of analysis, running PBS alone (filtered with 0.1 mm filter) leads to very limited background signal in both FSC-PMT and fluorescent channels and very few events could be recorded (Fig. 1f). Two JUNV-A647 positive populations, which we will refer to as ...
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NA 1.45 oil-immersion objective lens with an excitation power of 50 W/cm2. The fluorescence signal was collected by the same objective, separated from the excitation light by a dichroic (ZT 405/532 rpc), filtered by an emission filter (FF01-480/40/25) and focused onto an EMCCD camera...